Exercise significantly affects the body of both animals and humans, including the composition of the digestive microbiome. This study aimed to determine the changes in the composition of the most numerous bacterial phyla (Firmicutes and Bacteroidetes, as well as the level of the Lactobacillaceae family) in the digestive system of horses under the influence of physical effort. The study included a group of 17 Thoroughbred racehorses at the age of 3 years, fed the same forage, from whom feces samples were collected individually before and 48 h after physical effort. The obtained samples were subjected to DNA isolation and RT-PCR analysis. The results showed a significant increase in the level of both phyla after exercise compared to the state before physical effort; there were no such differences in the level of facultative aerobes, i.e., the Lactobacillaceae family (although a decreasing tendency was found after exercise). In addition, the analysis of the level of the studied phyla indicates individual differences in horses’ response to the effort.

中文翻译：

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剧烈运动对纯种赛马消化系统中拟杆菌和硬毛藻水平的影响

运动会显着影响动物和人类的身体，包括消化微生物组的组成。这项研究的目的是确定大多数细菌门（Firmicutes和Bacteroidetes）的组成以及乳杆菌科的水平变化。家庭）在身体消化力的影响下在马的消化系统中。该研究包括一组17只3岁的纯种赛马，他们饲喂相同的草料，在进行体力锻炼之前和之后48小时分别收集了粪便样本。对获得的样品进行DNA分离和RT-PCR分析。结果表明，与体育锻炼之前的状态相比，运动后两种门的水平均显着增加。兼性需氧菌，即乳酸杆菌科的水平没有这种差异（尽管运动后发现下降的趋势）。此外，对所研究门的水平的分析表明，马对这种努力的反应存在个体差异。