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Roles of Porphyromonas gulae proteases in bacterial and host cell biology
Cellular Microbiology ( IF 3.4 ) Pub Date : 2021-01-24 , DOI: 10.1111/cmi.13312
Alam Saki Urmi 1 , Hiroaki Inaba 1 , Ryota Nomura 2 , Sho Yoshida 1 , Naoya Ohara 3 , Fumitoshi Asai 4 , Kazuhiko Nakano 2 , Michiyo Matsumoto-Nakano 1
Affiliation  

Porphyromonas gulae, an animal-derived periodontal pathogen, expresses several virulence factors, including fimbria, lipopolysaccharide (LPS) and proteases. We previously reported that its invasive efficiency was dependent on fimbriae types. In addition, P. gulae LPS increased inflammatory responses via toll-like receptors. The present study was conducted to investigate the involvement of P. gulae proteases in bacterial and host cell biology. Porphyromonas gulae strains showed an ability to agglutinate mouse erythrocytes and also demonstrated co-aggregation with Actinomyces viscosus, while the protease inhibitors antipain, PMSF, TLCK and leupeptin diminished P. gulae proteolytic activity, resulting in inhibition of haemagglutination and co-aggregation with A. viscosus. In addition, specific proteinase inhibitors were found to reduce bacterial cell growth. Porphyromonas gulae inhibited Ca9-22 cell proliferation in a multiplicity of infection- and time-dependent manner. Additionally, P. gulae-induced decreases in cell contact and adhesion-related proteins were accompanied by a marked change in cell morphology from well spread to rounded. In contrast, inhibition of protease activity prevented degradation of proteins, such as E-cadherin, β-catenin and focal adhesion kinase, and also blocked inhibition of cell proliferation. Together, these results indicate suppression of the amount of human proteins, such as γ-globulin, fibrinogen and fibronectin, by P. gulae proteases, suggesting that a novel protease complex contributes to bacterial virulence.

中文翻译:

Porphyromonas gulae 蛋白酶在细菌和宿主细胞生物学中的作用

Porphyromonas gulae是一种动物源性牙周病原体,表达多种毒力因子,包括菌毛、脂多糖 (LPS) 和蛋白酶。我们之前报道过它的侵入效率取决于菌毛类型。此外,P. gulae LPS 通过 toll 样受体增加炎症反应。本研究旨在调查P. gulae蛋白酶在细菌和宿主细胞生物学中的作用。Porphyromonas gulae菌株表现出凝集小鼠红细胞的能力,并且还表现出与粘放线菌的共聚集,而蛋白酶抑制剂antipain、PMSF、TLCK 和 leupeptin 减少了P. gulae蛋白水解活性,导致抑制血凝和与A. viscosus的共聚集。此外,发现特定的蛋白酶抑制剂可以减少细菌细胞的生长。Porphyromonas gulae以多种感染和时间依赖性方式抑制 Ca9-22 细胞增殖。此外,P. gulae-诱导的细胞接触和粘附相关蛋白的减少伴随着细胞形态从良好扩散到圆形的显着变化。相比之下,蛋白酶活性的抑制阻止了蛋白质的降解,例如 E-钙粘蛋白、β-连环蛋白和粘着斑激酶,并且还阻止了对细胞增殖的抑制。总之,这些结果表明P. gulae蛋白酶抑制了人类蛋白质的数量,例如 γ-球蛋白、纤维蛋白原和纤连蛋白,这表明一种新的蛋白酶复合物有助于细菌毒力。
更新日期:2021-01-24
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