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Hydroxyapatite formation with the interface of chitin and chitosan
Journal of the Taiwan Institute of Chemical Engineers ( IF 5.7 ) Pub Date : 2021-01-23 , DOI: 10.1016/j.jtice.2021.01.004
Chen-Hsueh Lin , Yu-Sheng Chen , Wei-Lun Huang , Tzung-Chin Hung , Ten-Chin Wen

Hydroxyapatite (HA) film atop artificial implants surface can provide bioactive, osteoconductive and bone-similar interface. Traditional technique utilizes collagen coating to induce HA mineralization via charged functional groups. However, collagen loss as aging might lead to unstable interface. In this study, we presume that chitin (CH) with acetamide group chelates calcium ion and chitosan (CS) with amine group chelates phosphate ion, providing an ambiance for the HA crystallite formation. The presumption is evidenced by molar conductivity experiments. Besides, the hydrogen bonds of CH and electrostatic repulsion force of CS render different molecular architecture for the tunnel-connected structure. Furthermore, it forms porous morphology after HA mineralization evidenced by SEM images. Interestingly, mixing CH and CS provides the interface for Ca2+/PO43- capturing, rendering the formation of micro-crystallite with the 1.69/1.7 for the ratio of calcium to phosphate, being evidenced by EDX and AES experiments. Moreover, crystallinity index is calculated with XRD and FTIR spectra that CH–CS–HA possesses the highest crystallite to amorphous ratio. The HA film also shows good biocompatibility through L929 MTT test with 100% cell viability for the potential bio-applications. By the tapping-mode of AFM, the Young's modulus of CH–CS–HA surface is estimated to be 2.04 GPa near that of native bone.



中文翻译:

具有几丁质和壳聚糖界面的羟基磷灰石形成

人造植入物表面上的羟基磷灰石(HA)膜可提供生物活性,骨传导性和骨相似界面。传统技术利用胶原蛋白涂层通过带电的官能团诱导HA矿化。但是,胶原蛋白由于老化而损失可能会导致界面不稳定。在这项研究中,我们假设带有乙酰胺基的几丁质(CH)螯合钙离子和带有胺基的壳聚糖(CS)螯合磷酸根离子,为HA微晶的形成提供了氛围。摩尔电导率实验证明了这一推测。此外,CH的氢键和CS的静电排斥力为隧道连接结构提供了不同的分子结构。此外,在HA矿化后,通过SEM图像证明它形成了多孔形态。有趣的是EDX和AES实验证明,捕获2+ / PO 4 3-可以形成钙微比为1.69 / 1.7的微晶。此外,通过XRD和FTIR光谱计算得出结晶度指数,表明CH–CS–HA具有最高的微晶与非晶态比。HA膜还通过L929 MTT测试显示出良好的生物相容性,对于潜在的生物应用具有100%的细胞活力。通过原子力显微镜的敲击模式,CH–CS–HA表面的杨氏模量估计接近天然骨2.04 GPa。

更新日期:2021-02-05
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