Screening for the Rodentibacter species is part of the microbiologic quality assurance programs of laboratory rodents all over the world. Nevertheless, currently there are no PCR amplification techniques available for the diagnostic of R. ratti, R. heidelbergensis and of a Rodentibacter related β-haemolytic taxon. The aim of this study was to utilize the differences in the sequence of the Internal Transcribed Spacer (ITS) regions of R. pneumotropicus, R. heylii, R. ratti, R. heidelbergensis and of the β-haemolytic Rodentibacter taxon for the design of specific PCR assays for these species. The ITS^ile+ala sequence variations allowed the design of specific forward and reverse primers for each species included, that could be combined in different multiplex assays.

The performance characteristics specificity and sensitivity registered for each primer pair against a diverse collection of Pasteurellaceae isolated from rats and mice and of further non-Pasteurellaceae strains was 100% for all five Rodentibacter species included. In addition, the PCR assays displayed high limits of detection and could be successfully used for detection of Rodentibacter spp. DNA in clinical swabs of laboratory mice and rats.

Overall, the assays described here represent the first PCRs able to diagnose R. ratti, R. heidelbergensis and the β-haemolytic Rodentibacter taxon, whose diagnostic to species level could further facilitate better understanding of their geographic distribution, prevalence, and biology in the future.

Rodentibacter种类的筛选是世界各地实验室啮齿动物微生物质量保证计划的一部分。不过，目前有可用于诊断无PCR扩增技术R. RATTI，R.海德堡和的Rodentibacter相关β溶血性类群。本研究的目的是利用内转录间隔区的序列中的差异（ITS）的区域R. pneumotropicus，R. heylii，R. RATTI，R.海德堡和β溶血的Rodentibacter分类单元用于设计这些物种的特异性 PCR 检测。智能交通系统^ile+ala序列变异允许为每个物种设计特定的正向和反向引物，可以在不同的多重检测中组合。

对于从大鼠和小鼠中分离的各种巴斯德氏菌属以及其他非巴斯德氏菌属菌株，每个引物对登记的性能特征特异性和灵敏度对于包括的所有五种啮齿类杆菌属均为 100% 。此外，PCR 检测显示出较高的检测限，可成功用于啮齿类杆菌属的检测。实验室小鼠和大鼠临床拭子中的 DNA。

总的来说，这里描述的检测代表了第一批能够诊断R. ratti、R. heidelbergensis和 β-溶血性啮齿类杆菌分类群的PCR，其对物种水平的诊断可以进一步促进更好地了解它们的地理分布、流行率和未来的生物学.