Objective

To assess the role of embryo secretome in modifying the molecular profile of glycodelin A (GdA) in endometrial organoids (ORG) mimicking the implantation window. To verify whether the use of embryo-conditioned culture medium at the time of the embryo transfer may increase in vitro fertilization outcome.

Design

Molecular study with human endometrial ORG and embryo-conditioned culture medium. Retrospective study using prospectively recorded data.

Setting

University hospital.

Patient(s)

For isolation and culture of endometrial glandular ORG, endometrial biopsy specimens from five white women of proven fertility undergoing laparoscopy for tubal sterilization. A total of 75 women undergoing intracytoplasmic sperm injection for tubal and/or male infertility factor.

Interventions(s)

In vitro fertilization.

Main Outcome Measure(s)

Pinopodes presence in human endometrial ORG. Glycodelin A expression profile by means of two-dimensional electrophoresis. In vitro fertilization outcome.

Result(s)

This in vitro study demonstrated that the treatment of endometrial ORG with the secretome of medium conditioned by the growing embryo increased the GdA relative abundance and induced a different glycoform pattern. Biochemical and clinical pregnancy rate significantly increased when the spent medium was loaded during the transfer (17.5% vs. 36.6% and 16.5% vs. 35.1%, respectively).

Conclusion(s)

This study demonstrated that the secretome of implanting embryos is able to induce the expression as well as to determine the relative abundance and the glycosilation profile of endometrial GdA, a protein having a key role in the embryo-endometrial cross talk. Moreover, a significant increase in pregnancy rate was observed when the embryo transfer was performed by using the culture medium conditioned by the growing embryo.

中文翻译：

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胚胎分泌组在植入部位旁分泌通讯中的新兴作用：概念证明

客观的

评估胚胎分泌组在模拟植入窗口的子宫内膜类器官 (ORG) 中改变糖苷脂 A (GdA) 分子谱中的作用。验证在胚胎移植时使用胚胎条件培养基是否可以增加体外受精结果。

设计

人类子宫内膜 ORG 和胚胎条件培养基的分子研究。使用前瞻性记录数据的回顾性研究。

环境

大学医院。

耐心）

为了分离和培养子宫内膜腺体 ORG，来自五名已证实生育能力的白人女性接受腹腔镜输卵管绝育手术的子宫内膜活检标本。共有 75 名女性因输卵管和/或男性不育因素而接受卵胞浆内单精子注射。

干预措施

体外受精。

主要观察指标）

Pinopodes 存在于人类子宫内膜 ORG。Glycodelin A 通过二维电泳的表达谱。体外受精结果。

结果）

这项体外研究表明，用受生长胚胎调节的培养基分泌组处理子宫内膜 ORG 增加了 GdA 相对丰度并诱导了不同的糖型模式。在转移过程中装载用过的培养基时，生化和临床妊娠率显着增加（分别为 17.5% 对 36.6% 和 16.5% 对 35.1%）。

结论

该研究表明，植入胚胎的分泌组能够诱导表达以及确定子宫内膜 GdA 的相对丰度和糖基化谱，GdA 是一种在胚胎 - 子宫内膜串扰中起关键作用的蛋白质。此外，当使用由生长胚胎调节的培养基进行胚胎移植时，观察到妊娠率显着增加。