Lipase-mediated transesterification is a greener method for renewable biodiesel production, which can maintain high-level purity of the end products compared to the chemical transesterification methods. Hence, in this study, lipase was produced indigenously using Solid State Fermentation (SSF) from Rhizopus oryzae NRRL 3562, which was further immobilized on activated celite for transesterification. The evaluation of various transesterification attributes and stepwise optimizations were carried out for two different feedstock lipids, viz., a unialgal culture of Chlorella minutissima MCC 27, and a phyco-myco co-culture of C. minutissima MCC 27 and Aspergillus awamori. The optimal conditions, viz., 1:3–1:4 oil:methanol (molar ratio), 15 U lipase, 35 °-40 °C reaction temperature, 1% initial water loading, 1:0.4–1:0.6 substrate:hexane (w/v), 200–250 rpm agitation speed, and 30 h reaction duration ensued 91.52–93.25% fatty acid methyl ester (FAME) conversions in the selected microbial strains. The spent lipase showed potential for cyclic use and was found to have upheld almost 90% residual activity at the end of the 5^th cycle. The two resultant biodiesels showed comparable fuel properties except a marginal alleviation in density, viscosity, and iodine value in case of C. minutissima MCC 27 and A. awamori co-culture biodiesel compared to C. minutissima biodiesel.

脂肪酶介导的酯交换反应是一种可再生生物柴油生产的绿色方法，与化学酯交换方法相比，它可以保持最终产品的高纯度。因此，在本研究中，脂肪酶是使用米根霉NRRL 3562的固态发酵（SSF）本地生产的，并进一步固定在活化的硅藻土上进行酯交换。各种酯交换属性和逐步优化的评价进行了对于两种不同的原料的脂质，即，一个unialgal培养小球藻minutissima MCC 27和的Phyco对于-MYCO共培养C. minutissima MCC 27和泡盛曲霉。最佳条件，即1：3-1：4油：甲醇（摩尔比），15 U脂肪酶，35°-40°C反应温度，1％初始水负载，1：0.4-1：0.6底物：在选定的微生物菌株中，使用己烷（w / v），200–250 rpm的搅拌速度和30小时的反应时间可确保91.52–93.25％的脂肪酸​​甲酯（FAME）转化率。用过的脂肪酶显示出可循环使用的潜力，并在第5^个循环结束时发现其具有近90％的残留活性。与C. minutissima生物柴油相比，在C. minutissima MCC 27和A. awamori共培养生物柴油的情况下，除了密度，粘度和碘值的边际缓解有所降低外，两种最终的生物柴油均具有可比的燃料特性。