Metazachlor belongs to one of the most used herbicides throughout the world. In order to prevent the contamination of water bodies by such herbicides, the riparian buffers are constructed. The selection of appropriate plant species for this purpose is necessary. In our project, we studied the possibility of grey poplar to uptake and biotransform metazachlor, along with the phytotoxic effect of metazachlor and its metabolites. We used two different models – suspension cultures and poplar regenerants cultivated in vitro. Our results show that the herbicide metazachlor is readily metabolized by both suspension cultures and regenerants to 16 detectable metabolites. The detailed scheme of biotransformation pathway in poplar tissue is presented for the first time. The profile of detected metabolites was approximately the same in poplar cell cultures and regenerants, but the ratio and amounts of particular compounds was significantly different. Generally, the highest concentration (peak area/mg of DW) of all metabolites was present in the roots; the only exception was lactate conjugate (deCl-MZCl-Lact), which accumulated in the cultivation media. Although the plants were not visibly affected by metazachlor or its metabolites, they showed changes in activity of antioxidant enzymes and increased content of phenolic substances, the indicators of stress.

甲草胺属于全世界使用最广泛的除草剂之一。为了防止此类除草剂污染水体，构建了河岸缓冲带。为此目的，必须选择合适的植物。在我们的项目中，我们研究了灰杨吸收和代谢草胺的可能性，以及草胺及其代谢产物的植物毒性作用。我们使用了两种不同的模型–悬浮培养和体外培养的杨树再生剂。我们的结果表明，除草剂灭草灵很容易被悬浮培养物和再生剂代谢成16种可检测的代谢物。首次提出了杨树组织中生物转化途径的详细方案。在杨树细胞培养物和再生剂中，检测到的代谢物的分布图大致相同，但是特定化合物的比例和数量却有显着差异。通常，所有代谢产物的最高浓度（峰面积/ mg DW）都存在于根中。唯一的例外是乳酸共轭物（deCl-MZCl-Lact），其在培养基中积累。尽管这些植物不受苯甲草胺或其代谢产物的明显影响，但它们显示出抗氧化剂酶活性的变化和酚类物质（胁迫指标）的含量增加。