Gastric cancer treatment remains a major challenge. There are many reports on the positive efficacy of Bacteriocins associated with anti-cancer sequences. The main purpose of this study was to determine and design a fusion gene contraceptive containing anticancer Enterocin A, R-type pyocin, Lactocin, ligand against AGS gastric cancer cell line using Real Time_ RT_ PCR technique. This study was designed EntA-PynR-Lac recombinant proteins containing ligand and anticancer using bioinformatics software. Escherichia coli BL-21 was used to express cloned genes in expression vectors that have a T7 bacteriophage promoter. The specific ligand of the AGS cell line was designed and added to the recombinant construct sequence, and its three-dimensional structures, conformity, and stability were evaluated. Synthesis of fusion gene concentrate was performed in the expression vector, the transformation of the recombinant plasmid containing anticancer fusion gene construct in the respective host. Confirmation and purification of the recombinant protein by Western blotting and nickel column chromatography through the His tag, cytotoxicity determination against AGS cell line via the MTT technique, and finally, Real-time PCR was performed. Protein fusion at a concentration of 80 μg/ml in 24 h kills 83% of the cells tested and treatment of the cells with this concentration leads to increased expression of caspase3 and bax genes and decreased expression of bcl2. The results show that the treatment of gastric cancer cells with recombinant protein at a concentration of 80 μg/ml induces apoptosis in this cell line.

胃癌治疗仍然是主要挑战。关于抗癌序列的细菌素的阳性疗效有许多报道。这项研究的主要目的是使用RealTime_RT_PCR技术确定和设计一种融合基因避孕药，其中包含抗癌肠球菌素A，R型霉素，乳球菌素，抗AGS胃癌细胞系的配体。本研究使用生物信息学软件设计了包含配体和抗癌剂的EntA-PynR-Lac重组蛋白。大肠杆菌BL-21cDNA用于在具有T7噬菌体启动子的表达载体中表达克隆的基因。设计AGS细胞系的特异性配体并将其添加到重组构建体序列中，并评估其三维结构，整合性和稳定性。在表达载体中进行融合基因浓缩物的合成，在各自的宿主中转化含有抗癌融合基因构建体的重组质粒。通过蛋白质印迹法和His标签通过镍柱色谱法对重组蛋白进行确认和纯化，通过MTT技术确定对AGS细胞系的细胞毒性，最后进行实时PCR。caspase3和bax基因与bcl2表达降低。结果表明，用浓度为80μg/ ml的重组蛋白处理胃癌细胞会诱导该细胞系凋亡。