Applied Biochemistry and Biotechnology ( IF 3 ) Pub Date : 2021-01-23 , DOI: 10.1007/s12010-021-03492-1 Jianfei Wang 1 , Shaoming Jiang 1 , Jiaqi Huang 1, 2 , Huanyu Guo 1 , Xudong Bi 1, 3 , Maolin Hou 1, 4 , Xingyu Chen 1 , Shibo Hou 1 , Hebei Lin 1 , Yuming Lu 1 , Hujie Lv 1 , Jinyue Qiao 1 , Ruiyi Yang 1 , Shijie Liu 1
In this study, Box-Behnken design was applied to optimize the initial concentrations of 4 cations for L-lactic acid production from fructose by homologous batch fermentation of Lactobacillus pentosus cells. The optimum initial cation concentrations were obtained as 6.542 mM Mg2+, 3.765 mM Mn2+, 2.397 mM Cu2+, and 3.912 mM Fe2+, respectively. The highest L-lactic acid yield and productivity were obtained as 0.935 ± 0.005 g/g fructose and 1.363 ± 0.021 g/(L × h), respectively, with a maximum biomass concentration of 7.97 ± 0.17 g/L. The effectiveness of the optimization by Box-Behnken design was confirmed based on the small errors between predicted results and experimental results shown as 0.3%, − 0.2%, and − 1.2%, respectively. The quadratic models with high accuracy and reliability can be applied to mathematically forecasted the fermentation performance. After the optimization, the lactic acid yield and productivity were significantly improved by 3.7% and 21.0%, respectively.
中文翻译:
戊糖乳杆菌细胞从果糖生产 L-乳酸的初始阳离子浓度优化
在本研究中,应用 Box-Behnken 设计优化戊糖乳杆菌细胞同源分批发酵从果糖生产 L-乳酸的 4 种阳离子的初始浓度。获得的最佳初始阳离子浓度为 6.542 mM Mg 2+、3.765 mM Mn 2+、2.397 mM Cu 2+和 3.912 mM Fe 2+, 分别。最高的 L-乳酸产量和生产力分别为 0.935 ± 0.005 g/g 果糖和 1.363 ± 0.021 g/(L × h),最大生物量浓度为 7.97 ± 0.17 g/L。Box-Behnken 设计优化的有效性基于预测结果和实验结果之间的小误差得到证实,分别显示为 0.3%、-0.2% 和-1.2%。具有高精度和可靠性的二次模型可用于对发酵性能进行数学预测。优化后,乳酸收率和生产率分别显着提高了3.7%和21.0%。