当前位置: X-MOL 学术RSC Adv. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Controlled “off–on” fluorescent probe for the specific detection of hyperhomocysteinemia
RSC Advances ( IF 3.9 ) Pub Date : 2021-1-22 , DOI: 10.1039/d0ra08710f
Jinrong Zheng 1 , Jianlong Li 2 , Hongli Luo 3 , Lingbin Sun 4 , Mangmang Sang 1 , Xiu Yu 5
Affiliation  

Hyperhomocysteinemia is an established risk factor for atherosclerosis and vascular disease. Therefore, designing a hyperhomocysteinemia specific probe is of great significance for the early warning of cardiovascular diseases. However, developing probes that can efficiently and specifically recognize homocysteine (Hcy) remains a tremendous challenge. Therefore, we designed an Hcy-specific fluorescent probe (HSFP) with excellent selectivity and anti-interference capability. Interestingly, this probe can automatically “off–on” in water solution, but the fluorescence of HSFP remains “off” when Hcy is present in the solution. The spectroscopic data demonstrated that the fluorescence of HSFP attenuated 13.8 folds toward Hcy in water without interference from other biothiols and amino acids. Furthermore, HSFP can sensitively reflect the change of Hcy content in cells. Therefore, HSFP was further applied to detect hyperhomocysteinemia in vivo with high efficiency. In summary, we have developed an Hcy-specific fluorescent probe to efficiently detect Hcy in vivo and in vitro, which may contribute to basic or clinical research.

中文翻译:

用于特异性检测高同型半胱氨酸血症的受控“关-开”荧光探针

高同型半胱氨酸血症是动脉粥样硬化和血管疾病的既定危险因素。因此,设计高同型半胱氨酸血症特异性探针对于心血管疾病的早期预警具有重要意义。然而,开发能够有效且特异性地识别同型半胱氨酸 (Hcy) 的探针仍然是一个巨大的挑战。因此,我们设计了一种具有优异选择性和抗干扰能力的Hcy特异性荧光探针(HSFP)。有趣的是,这种探针在水溶液中可以自动“关闭-打开”,但当溶液中存在 Hcy 时,HSFP 的荧光仍然“关闭”。光谱数据表明,HSFP 的荧光在水中向 Hcy 衰减 13.8 倍,而不受其他生物硫醇和氨基酸的干扰。此外,HSFP可以灵敏地反映细胞内Hcy含量的变化。因此,HSFP 被进一步应用于检测高同型半胱氨酸血症体内高效。总之,我们开发了一种Hcy特异性荧光探针,可以在体内体外有效检测Hcy ,这可能有助于基础或临床研究。
更新日期:2021-01-22
down
wechat
bug