Obesity, characterized by expansion and metabolic dysregulation of white adipose tissue (WAT), has reached pandemic proportions and acts as a primer for a wide range of metabolic disorders. Remodelling of WAT lipidome in obesity and associated comorbidities can explain disease etiology and provide valuable diagnostic and prognostic markers. To support understanding of WAT lipidome remodelling at the molecular level, we performed in-depth lipidomics profiling of human subcutaneous and visceral WAT of lean and obese individuals. Tissue-tailored preanalytical and analytical workflows allowed accurate identification and semi-absolute quantification of 1636 and 737 lipid molecular species, respectively, and summarized here in a form of human WAT reference lipidome. Deep lipidomic profiling allowed to identify main lipid (sub)classes undergoing depot/phenotype specific remodelling. Furthermore, previously unanticipated diversity of WAT ceramides was uncovered. AdipoAtlas reference lipidome will serve as a data-rich resource for the development of WAT-specific high-throughput methods and as a scaffold for systems medicine data integration.

中文翻译：

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AdipoAtlas：人类白色脂肪组织的参考脂质组。

以白色脂肪组织（WAT）的扩张和代谢失调为特征的肥胖症已达到大流行的比例，并成为各种代谢疾病的引物。肥胖和相关合并症中WAT脂质组的重塑可以解释疾病的病因，并提供有价值的诊断和预后指标。为了支持在分子水平上对WAT脂质组重塑的理解，我们对瘦弱和肥胖个体的人皮下和内脏WAT进行了深入的脂质组学分析。量身定制的分析和分析工作流程可分别对1636和737种脂质分子种类进行准确鉴定和半绝对定量，并在此以人类WAT参考脂质组的形式进行了总结。深入的脂质组分析可确定经历库/表型特异性重塑的主要脂质（亚类）。此外，还发现了WAT神经酰胺以前无法预料的多样性。AdipoAtlas参考脂质组将作为开发WAT特定的高通量方法的丰富数据资源，并作为系统医学数据集成的支架。