We report perhaps the most comprehensive study of subsets of CD4+ and CD8+ and subsets of B cells in a mild symptomatic SARS-CoV-2+ immunocompetent patient and a common variable immunodeficiency disease (CVID) patient who had normal absolute lymphocyte counts and remained negative for SARS-CoV-2 IgG antibodies. Naïve (T_N), central memory (T_CM), effector memory (T_EM), and terminally differentiated effector memory (T_EMRA) subsets of CD4+ and CD8+ T cells, subsets of T follicular helper cells (cT_FH, T_FH1, T_FH2, T_FH17, T_FH1/T_FH17, and T_FR), CD4 Treg, CD8 Treg, mature B cells, transitional B cells, marginal zone B cells, germinal center (GC) B cells, CD21^low B cells, antibody-secreting cells (plasmablasts), and Breg cells were examined in patients and age-matched controls with appropriate monoclonal antibodies and isotype controls using multicolor flow cytometry. Different patterns of abnormalities (often contrasting) were observed in the subsets of CD4+ T, CD8+ T, B-cell subsets, and regulatory lymphocytes among the immunocompetent patient and CVID patient as compared to corresponding healthy controls. Furthermore, when data were analyzed between the 2 patients, the immunocompetent patient demonstrated greater changes in various subsets as compared to the CVID patient. These data demonstrate different immunological responses to SARS-CoV-2 infection in an immunocompetent patient and the CVID patient. A marked decrease in GC B cells and plasmablasts may be responsible for failure to make SARS-CoV-2 antibodies. The lack of SARS-CoV-2 antibodies with mild clinical disease suggests an important role of T-cell response in defense against SARS-CoV-2 infection.
Int Arch Allergy Immunol

中文翻译：

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存在体液免疫缺陷时 SARS-CoV-2 相关的 T 细胞反应

我们报告的可能是最全面的研究，针对一名症状轻微的 SARS-CoV-2+ 免疫功能正常的患者和一名普通变异型免疫缺陷病 (CVID) 患者的 CD4+ 和 CD8+ 亚群以及 B 细胞亚群，该患者的绝对淋巴细胞计数正常，且检测结果呈阴性。 SARS-CoV-2 IgG 抗体。幼稚 (T _N )、中枢记忆 (T _CM )、效应记忆 (TEM ₎和终末分化效应记忆 ( _TEMRA ) CD4+ 和 CD8+ T 细胞亚群、滤泡辅助 T 细胞亚群 (cT _FH、 T _FH 1 、T _FH 2、T _FH 17、T _FH 1/T _FH 17 和 T _FR )、CD4 Treg、CD8 Treg、成熟 B 细胞、移行 B 细胞、边缘区 B 细胞、生发中心 (GC) B 细胞、CD21使用多色流式细胞术，使用适当的单克隆抗体和同型对照对患者和年龄匹配的对照进行^{低B 细胞、抗体分泌细胞（浆母细胞）和 Breg 细胞的检查。}与相应的健康对照相比，在免疫功能正常的患者和 CVID 患者的 CD4+ T、CD8+ T、B 细胞亚群和调节性淋巴细胞亚群中观察到不同的异常模式（通常是对比）。此外，当对 2 名患者之间的数据进行分析时，与 CVID 患者相比，免疫功能正常的患者在各个亚群中表现出更大的变化。这些数据表明，免疫功能正常的患者和 CVID 患者对 SARS-CoV-2 感染的免疫反应不同。GC B 细胞和浆母细胞的显着减少可能是无法制造 SARS-CoV-2 抗体的原因。轻度临床疾病缺乏 SARS-CoV-2 抗体表明 T 细胞反应在防御 SARS-CoV-2 感染中发挥着重要作用。
Int Arch 过敏免疫