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Peperomin E Induces Apoptosis and Cytoprotective Autophagy in Human Prostate Cancer DU145 Cells In Vitro and In Vivo
Planta Medica ( IF 2.7 ) Pub Date : 2021-01-21 , DOI: 10.1055/a-1348-1634 Min Lin 1 , Qiannan Zhu 1 , Yunzhi Li 1, 2 , Jigang Pan 3
Planta Medica ( IF 2.7 ) Pub Date : 2021-01-21 , DOI: 10.1055/a-1348-1634 Min Lin 1 , Qiannan Zhu 1 , Yunzhi Li 1, 2 , Jigang Pan 3
Affiliation
Peperomin E was first isolated from Peperomia dindygulensis, an anticarcinogenic herb, and exhibited anticancer activity in many cancer cell lines. To date, it is unknown whether peperomin E has an effect on human prostate cancer DU145 cells in vitro and in vivo. In this study, we used MTT to assess the proliferation inhibition activity of peperomin E in DU145 cells in vitro and observed the cell morphological changes by a phase contrast microscope. A DU145 cell xenograft tumor mouse model was used to evaluate the efficacy of peperomin E in vivo. Apoptosis rates were measured by flow cytometry, and protein expression levels were analyzed by western blot. The results showed that peperomin E significantly inhibited the proliferation of DU145 cells in vitro and reduced the weight and volume of tumors in vivo. Peperomin E also significantly induced the apoptosis and autophagic response of DU145 cells. The autophagic inhibitors LY294002 and chloroquine enhanced peperomin E-mediated inhibition of DU145 cell proliferation and induction of DU145 cell apoptosis. The results also showed that the Akt/mTOR pathway participated in peperomin E-induced autophagy in DU145 cells. In summary, our finding showed that peperomin E had an effect on DU145 cells in vitro and in a nude mouse DU145 cell xenograft model in vivo, demonstrated that peperomin E could significantly induce apoptosis and the autophagic response in DU145 cells and that autophagy played a cytoprotective role in peperomin E-treated DU145 cells. These results suggest that the combination of peperomin E treatment and autophagic inhibition has potential for the treatment of prostate cancer.
中文翻译:
Peperomin E 在体外和体内诱导人前列腺癌 DU145 细胞的凋亡和细胞保护性自噬
Peperomin E 最早是从 Peperomia dindygulensis(一种抗癌药草)中分离出来的,并在许多癌细胞系中表现出抗癌活性。迄今为止,peperomin E 是否在体外和体内对人前列腺癌 DU145 细胞有影响尚不清楚。本研究采用MTT法评估peperomin E对DU145细胞的体外增殖抑制活性,并通过相差显微镜观察细胞形态变化。DU145 细胞异种移植肿瘤小鼠模型用于评估peperomin E 在体内的功效。通过流式细胞术测量细胞凋亡率,并通过蛋白质印迹分析蛋白质表达水平。结果表明peperomin E在体外显着抑制DU145细胞的增殖,在体内降低肿瘤的重量和体积。Peperomin E 还显着诱导 DU145 细胞的凋亡和自噬反应。自噬抑制剂LY294002和氯喹增强了peperomin E介导的DU145细胞增殖抑制和DU145细胞凋亡诱导。结果还表明Akt/mTOR通路参与了胡椒素E诱导的DU145细胞自噬。总之,我们的发现表明peperomin E在体外和裸鼠DU145细胞异种移植模型体内对DU145细胞有影响,证明peperomin E可以显着诱导DU145细胞的凋亡和自噬反应,自噬起到细胞保护作用。 peperomin E 处理的 DU145 细胞中的作用。这些结果表明peperomin E治疗和自噬抑制的组合具有治疗前列腺癌的潜力。
更新日期:2021-01-21
中文翻译:
Peperomin E 在体外和体内诱导人前列腺癌 DU145 细胞的凋亡和细胞保护性自噬
Peperomin E 最早是从 Peperomia dindygulensis(一种抗癌药草)中分离出来的,并在许多癌细胞系中表现出抗癌活性。迄今为止,peperomin E 是否在体外和体内对人前列腺癌 DU145 细胞有影响尚不清楚。本研究采用MTT法评估peperomin E对DU145细胞的体外增殖抑制活性,并通过相差显微镜观察细胞形态变化。DU145 细胞异种移植肿瘤小鼠模型用于评估peperomin E 在体内的功效。通过流式细胞术测量细胞凋亡率,并通过蛋白质印迹分析蛋白质表达水平。结果表明peperomin E在体外显着抑制DU145细胞的增殖,在体内降低肿瘤的重量和体积。Peperomin E 还显着诱导 DU145 细胞的凋亡和自噬反应。自噬抑制剂LY294002和氯喹增强了peperomin E介导的DU145细胞增殖抑制和DU145细胞凋亡诱导。结果还表明Akt/mTOR通路参与了胡椒素E诱导的DU145细胞自噬。总之,我们的发现表明peperomin E在体外和裸鼠DU145细胞异种移植模型体内对DU145细胞有影响,证明peperomin E可以显着诱导DU145细胞的凋亡和自噬反应,自噬起到细胞保护作用。 peperomin E 处理的 DU145 细胞中的作用。这些结果表明peperomin E治疗和自噬抑制的组合具有治疗前列腺癌的潜力。