With an aim of enhancing drought tolerance using a marker‐assisted backcrossing (MABC) approach, we introgressed the “QTL‐hotspot” region from ICC 4958 accession that harbors quantitative trait loci (QTLs) for several drought‐tolerance related traits into three elite Indian chickpea (Cicer arietinum L.) cultivars: Pusa 372, Pusa 362, and DCP 92‐3. Of eight simple sequence repeat (SSR) markers in the QTL‐hotspot region, two to three polymorphic markers were used for foreground selection with respective cross‐combinations. A total of 47, 53, and 46 SSRs were used for background selection in case of introgression lines (ILs) developed in genetic backgrounds of Pusa 372, Pusa 362, and DCP 92‐3, respectively. In total, 61 ILs (20 BC₃F₃ in Pusa 372; 20 BC₂F₃ in Pusa 362, and 21 BC₃F₃ in DCP 92‐3), with >90% recurrent parent genome recovery were developed. Six improved lines in different genetic backgrounds (e.g. BGM 10216 in Pusa 372; BG 3097 and BG 4005 in Pusa 362; IPC(L4‐14), IPC(L4‐16), and IPC(L19‐1) in DCP 92‐3) showed better performance than their respective recurrent parents. BGM 10216, with 16% yield gain over Pusa 372, has been released as Pusa Chickpea 10216 by the Central Sub‐Committees on Crop Standards, Notification and Release of Varieties of Agricultural Crops, Ministry of Agriculture and Farmers Welfare, Government of India, for commercial cultivation in India. In summary, this study reports introgression of the QTL‐hotspot for enhancing yield under rainfed conditions, development of several introgression lines, and release of Pusa Chickpea 10216 developed through molecular breeding in India.

中文翻译：

---

“ QTL-热点”区域的渗入提高了三种优良鹰嘴豆品种的耐旱性和籽粒产量

为了使用标记辅助回交（MABC）方法提高干旱耐受性，我们将ICC 4958入选的“ QTL热点”区域引入了具有几个耐旱相关性状的数量性状基因座（QTL）的区域，引入了三个印度精英地区鹰嘴豆（Cicer arietinum L.）品种：Pusa 372，Pusa 362和DCP 92-3。在QTL热点区域中的八个简单序列重复（SSR）标记中，有2-3个多态性标记用于具有各自交叉组合的前景选择。如果分别在Pusa 372，Pusa 362和DCP 92-3的遗传背景中形成了渗入系（IL），则总共使用47、53和46个SSR进行背景选择。总共61个IL（20 BC ₃ F₃在普萨372; 开发了Pusa 362中的20 BC ₂ F ₃和DCP 92-3中的21 BC ₃ F ₃，具有> 90％的复发亲本基因组回收率。在不同遗传背景下的六种改良品系（例如Pusa 372中的BGM 10216; Pusa 362中的BG 3097和BG 4005; DCP 92-3中的IPC（L4-14），IPC（L4-16）和IPC（L19-1） ）表现出比他们各自的经常性父母更好的表现。印度政府农业，农作物福利部作物标准，通知和发布品种分中央委员会将BGM 10216比Pusa 372增产16％，成为Pusa Chickpea 10216。印度的商业化种植。总而言之，这项研究报告了QTL热点，用于在雨水条件下提高产量，开发数个渗种系以及释放通过分子育种在印度开发的Pusa鹰嘴豆10216。