Artificial enzymes based on the modification of a protein structure for the creation of new active catalytic sites have experienced a great boom in recent years. Multidisciplinary strategies of genetic engineering, chemical or chemical biological tools have been successfully described to synthetize them. However, a challenge has been focused on the creating of artificial enzymes with more than one active site. This could represent a new direction in the application of enzymatic tools in sustainable chemistry. Actually, only a few technologies have been described for designing artificial enzymes with two or multiple active sites. This review article underlines these most significant advances.

近年来，基于蛋白质结构的修饰以产生新的活性催化位点的人造酶经历了巨大的发展。已经成功地描述了遗传工程，化学或化学生物学工具的多学科策略来合成它们。然而，挑战集中在创造具有一个以上活性位点的人造酶上。这可能代表了酶工具在可持续化学中的应用新方向。实际上，仅描述了几种设计具有两个或多个活性位点的人工酶的技术。这篇评论文章强调了这些最重要的进展。