Brewer's spent grain (BSG) is the major byproduct generated by the brewing industry. It has 50–70% fiber and 14–30% protein contents. This study investigates the technical and economic performances for producing protein-rich product (PP) from BSG using enzyme-assisted fractionation process. This was done through process optimization, scale-up verification, and techno-economic analysis (TEA). The experiment was conducted with varying enzyme (Alcalase) loadings and enzymatic hydrolysis times. The results showed that the optimal condition was using Alcalase loading of 5 μL/g with hydrolysis time of 1 h for achieving a high protein concentration (46%) in PP and protein separation efficiency (80%). Using the optimal condition, the scaled-up process resulted in a consistent PP composition and protein separation efficiency. The experimental conditions and results were input into process simulation model for determining the mass and energy flows, from which TEA is derived. For a processing plant with a capacity of 590 t wet BSG per day, the minimum selling price of PP (MSPP) to achieve a 5% return was determined to be 1044 USD/t. Sensitivity analysis revealed that Alcalase and BSG costs have the most effect on the MSPP. Besides, protein separation efficiency is an important processing parameter in determining the MSPP.

啤酒厂的废谷物（BSG）是啤酒工业产生的主要副产品。它具有50–70％的纤维和14–30％的蛋白质含量。这项研究调查了使用酶辅助分馏工艺从BSG生产富含蛋白质的产品（PP）的技术和经济性能。这是通过流程优化，规模扩大验证和技术经济分析（TEA）来完成的。在不同的酶（Alcalase）负载量和酶促水解时间下进行了实验。结果表明，最佳条件是使用Alcalase负载量为5μL/ g，水解时间为1 h，以实现PP中高蛋白浓度（46％）和蛋白分离效率（80％）。使用最佳条件，按比例放大的过程将导致一致的PP组成和蛋白质分离效率。将实验条件和结果输入到过程模拟模型中，以确定质量和能量流，从中得出TEA。对于一个日处理能力为590吨湿BSG的加工厂，确定实现5％回报率的PP（MSPP）的最低销售价格确定为1044美元/吨。敏感性分析显示，Alcalase和BSG成本对MSPP的影响最大。此外，蛋白质分离效率是确定MSPP的重要工艺参数。敏感性分析显示，Alcalase和BSG成本对MSPP的影响最大。此外，蛋白质分离效率是确定MSPP的重要工艺参数。敏感性分析显示，Alcalase和BSG成本对MSPP的影响最大。此外，蛋白质分离效率是确定MSPP的重要工艺参数。