The G-quadruplex structure formed by the c-myc gene sequence has attracted much attention due to its important physiological function in biology and wide application in nanotechnology. So far, probes capable of recognition of c-myc G-quadruplex with both high specificity and sensitivity are still scarce. This work presented a cyanine dye fluorescent probe named Cy-1, which has almost no fluorescence in aqueous solution, but showing more than 1000-fold fluorescence enhancement for recognizing c-myc G-quadruplex. Cy-1 also has good specificity and can selectively recognize c-myc G-quadruplex from other a variety of G-quadruplex and non-G-quadruplex structures. These properties make Cy-1 a promising probe for c-myc G-quadruplex recognition in nanotechnology or biology.

由c-myc基因序列形成的G-四链体结构由于其在生物学中的重要生理功能和在纳米技术中的广泛应用而备受关注。迄今为止，仍缺乏能够同时识别高特异性和高灵敏度的c-myc G-四链体的探针。这项工作提出了一种名为Cy-1的花青染料荧光探针，该探针在水溶液中几乎没有荧光，但是在识别c-myc G-四链体时显示出超过1000倍的荧光增强。Cy-1也具有良好的特异性，并且可以从其他各种G-四链体和非G-四链体结构中选择性识别c-myc G-四链体。这些特性使Cy-1成为纳米技术或生物学中c-myc G-四链体识别的有前途的探针。