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Agrobacterium tumefaciens-mediated transformation of Coniella granati
Journal of Microbiological Methods ( IF 2.2 ) Pub Date : 2021-01-22 , DOI: 10.1016/j.mimet.2021.106149
Hongbo Yuan 1 , Hui Hou 1 , Tianxiang Huang 1 , Zengqiang Zhou 1 , Hongtao Tu 1 , Li Wang 1
Affiliation  

Pomegranate fruit rot caused by Coniella granati is among the most devastating diseases threatening pomegranate production. The pathogenic mechanism of this pathogen remains largely unknown due to lack of genetic transformation method. Herein, we developed an approach to the Agrobacterium tumefaciens-mediated transformation (ATMT) of C. granati using a plasmid vector encoding the green fluorescent protein (GFP) and hygromycin resistance (Hyg) genes. This approach yielded C. granati transformants that exhibited uniform, stable green fluorescence. We further optimized this ATMT protocol, enabling us to achieve a transformation efficiency of up to 300 transformants per 0.5 cm2 mycelial plug. Together, we thus provide the first report of the stable transformation of C. granati, laying a foundation for future functional studies characterizing this economically important fungal pathogen.



中文翻译:

根癌农杆菌介导的 Coniella granati 转化

Coniella granati引起的石榴果实腐烂是威胁石榴生产的最具破坏性的疾病之一。由于缺乏遗传转化方法,该病原体的致病机制在很大程度上仍不清楚。在此,我们开发了一种使用编码绿色荧光蛋白 ( GFP ) 和潮霉素抗性 ( Hyg ) 基因的质粒载体对C. granati进行根癌农杆菌介导的转化 (ATMT) 的方法。这种方法产生了表现出均匀、稳定的绿色荧光的C. granati转化体。我们进一步优化了这个 ATMT 协议,使我们能够实现每 0.5 cm 高达 300 个转化体的转化效率2菌丝栓。因此,我们共同提供了C. granati稳定转化的第一份报告,为未来表征这种具有重要经济意义的真菌病原体的功能研究奠定了基础。

更新日期:2021-02-08
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