Lentil cultivation could be challenged by combined heat and drought stress in semi-arid regions. We used RNA-seq approach to profile transcriptome changes of Lens culinaris exposed to individual and combined heat and drought stresses. It was determined that most of the differentially expressed genes observed in response to combined stress, could not be identified by analysis of transcriptome exposed to corresponding individual stresses. Interestingly, this study results revealed that the expression of ribosome generation and protein biosynthesis and starch degradation pathways related genes were uniquely up-regulated under the combined stress. Although multiple genes related to antioxidant activity were up-regulated in response to all stresses, variation in types and expression levels of these genes under the combined stress were higher than that of individual stresses. Using this comparative approach, for the first time, we reported up-regulation of several TF, CDPK, CYP, and antioxidant genes in response to combined stress in plants.

半干旱地区的小扁豆种植可能会受到高温和干旱胁迫的挑战。我们使用 RNA-seq 方法来分析Lens culinaris的转录组变化暴露于单独的和联合的热和干旱胁迫。已确定，大多数响应于联合胁迫观察到的差异表达基因不能通过分析暴露于相应个体胁迫的转录组来鉴定。有趣的是，这项研究结果表明，核糖体生成和蛋白质生物合成以及淀粉降解途径相关基因的表达在联合胁迫下被独特地上调。尽管多个与抗氧化活性相关的基因在所有胁迫下都被上调，但在联合胁迫下这些基因的类型和表达水平的变化高于单个胁迫。使用这种比较方法，我们首次报告了几种 TF、CDPK、CYP、