A novel green eco-friendly turn-on FRET sensing strategy was developed for detection of ketorolac (KET). One-pot water-dispersed chitosan stabilized silver nanoparticles (AgNPs) solution was synthesized by complexation of micellized chitosan solution with silver nitrate at low temperature followed by subsequent reduction using sodium borohydride at 70 °C. The preparation method was mild and produced AgNPs solution stable for eight months at 4 °C. Owing to the spectral overlap of the fluorescent spectrum of chitosan and absorption spectrum of AgNPs, a novel sensitive turn-on FRET sensing strategy was established based on enhancement of chitosan fluorescence by electrostatic destabilization of the monodispersed AgNPs upon the addition of KET. The sensor solution was applied for the selective detection of ketorolac from 0.05 to 0.4 μM in injection dosage forms and spiked environmental water samples with no interference either of the KET photocatalyzed degradation product or other interfering structurally related or coexisting substances. The developed strategy showed promising results and could be applied practically for the detection of KET in injection dosage forms and environmental water.

开发了一种新型的绿色环保的开启式FRET传感策略，用于检测酮咯酸（KET）。通过在低温下将胶束化的壳聚糖溶液与硝酸银络合，然后在70°C下使用硼氢化钠进行还原，来合成一锅水分散的壳聚糖稳定化银纳米颗粒（AgNPs）溶液。制备方法温和，产生的AgNPs溶液在4°C下稳定八个月。由于壳聚糖荧光光谱和AgNPs吸收光谱的光谱重叠，基于添加KET的单分散AgNPs的静电去稳定作用提高了壳聚糖荧光，建立了一种新颖的灵敏的FRET传感策略。传感器溶液用于从0.05到0的酮咯酸的选择性检测。注射剂型中的浓度为4μM，加标的环境水样品不受KET光催化降解产物或其他干扰结构相关或共存物质的干扰。所开发的策略显示出令人鼓舞的结果，可实际用于注射剂型和环境水中的KET检测。