Porphyrins absorb strongly in the visible region and are also excellent fluorophores that emit in the visible region that make them excellent candidates for fluorescence sensing and in vivo imaging. This work describes the fluorescence determination of adenine using cobalt complex of a simple porphyrin. Tetraphenylporphyrin (TPP) and tetraphenylpophyrinatocobalt(II) (CoTPP) were synthesized and characterised. TPP on metallation with cobalt resulted in the red shift of fluorescence emission in the region 652 nm and 716 nm and showed an enhancement in the emission peaks with the addition of the nucleobase, adenine. CoTPP is found to be an efficient fluorescent sensor for adenine in DMF solvent. The fluorescence enhancement is due to the formation of the ground state complex formation between adenine and CoTPP, which is supported by experimental evidences from UV- visible spectra, time resolved fluorescence life time measurements etc. The detection limit of adenine was found to be 4.2 μM using the CoTPP fluorescent probe. The proposed sensor is found to be highly selective for adenine in presence of other nitrogen bases like guanine, cytosine, uracil, thymine, alanine, histidine etc. in 1:1 concentration.

卟啉在可见光区有很强的吸收性，也是在可见光区发射的极好的荧光团，这使它们成为荧光传感和体内成像的绝佳候选物。这项工作描述了使用简单卟啉的钴络合物对腺嘌呤进行荧光测定。合成并表征了四苯基卟啉 (TPP) 和四苯基卟啉钴 (II) (CoTPP)。与钴金属化的 TPP 导致荧光发射在 652 nm 和 716 nm 区域发生红移，并且随着核碱基腺嘌呤的加入，发射峰增强。CoTPP 被发现是 DMF 溶剂中腺嘌呤的有效荧光传感器。荧光增强是由于腺嘌呤和 CoTPP 之间形成基态复合物，这得到了来自紫外可见光谱、时间分辨荧光寿命测量等的实验证据的支持。使用 CoTPP 荧光探针发现腺嘌呤的检测限为 4.2 μM。发现所提出的传感器在其他氮碱基如鸟嘌呤、胞嘧啶、尿嘧啶、胸腺嘧啶、丙氨酸、组氨酸等以 1:1 浓度存在的情况下对腺嘌呤具有高度选择性。