Aflatoxin B1 (AFB1) is a natural carcinogenic contaminant of agricultural product regulated at parts per billion (ppb) level; therefore, a validated method for the analysis of AFB1 is extremely important. In this study, a high-performance thin-layer chromatography (HPTLC) method was standardized and validated with respect to linearity, sensitivity, precision, and accuracy for the quantification of AFB1. Three extraction techniques using different solvent systems, namely QuEChERS (quick, easy, cheap, effective, rugged and safe) method, Contaminants Branch method and Best Foods method were evaluated for AFB1 extraction efficiency in food commodities, wherein QuEChERS method using acetonitrile‒methanol (40:60, V/V) gave maximum recovery of AFB1 in all commodities. Maximum AFB1 was recovered in dried chilli sample (89.98%), whereas the least recovery was obtained in wheat sample (68.20%). The optimized HPTLC method with chloroform‒acetone (9:1, V/V) as the mobile phase showed a compact AFB1 band at R_F value of 0.36 ± 0.02. The linear regression analysis of AFB1 showed good linearity over 10, 20, 40, 60, 80, 100, 120 ng/spot with a regression value of 0.9923 and %RSD less than 1. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.133 ng/band and 3.434 ng/band, respectively. The optimized HPTLC method was further used to assess the AFB1 content in marketed samples of groundnut, corn, rice, wheat and dried chillies from the Mumbai region and it was found that 8 groundnut samples, 5 corn samples, 5 wheat samples, and 7 chilli samples out of 20 each had AFB1 concentration above Indian permissible regulatory limit of 30 ppb.

黄曲霉毒素B1（AFB1）是农产品的天然致癌污染物，其含量控制在十亿分之几（ppb）的水平；因此，一种经过验证的AFB1分析方法非常重要。在这项研究中，高性能薄层色谱法（HPTLC）已标准化，并在线性，灵敏度，精密度和准确性方面进行了验证，以定量AFB1。评价了三种使用不同溶剂系统的提取技术，即QuEChERS（快速，简便，廉价，有效，坚固，安全）方法，污​​染物分支方法和Best Foods方法对食品中AFB1的提取效率，其中QuEChERS方法使用乙腈/甲醇（ 40：60，V / V）可在所有商品中最大程度地回收AFB1。干辣椒样品中回收的AFB1最高（89.98％），而小麦样品中回收的AFB1最少（68.20％）。以氯仿‒丙酮（9：1，V / V）为流动相的优化的HPTLC方法显示了一个紧凑的AFB1谱带，R _F值为0.36±0.02。AFB1的线性回归分析显示在10、20、40、60、80、100、120 ng / spot上具有良好的线性，回归值为0.9923和％RSD小于1。检测限（LOD）和定量限（LOQ）分别为1.133 ng / band和3.434 ng / band。进一步使用优化的HPTLC方法评估孟买地区销售的花生，玉米，大米，小麦和干辣椒样品中的AFB1含量，发现有8个花生样品，5个玉米样品，5个小麦样品和7个辣椒20个样本中的每个样本的AFB1浓度均高于印度允许的30 ppb监管限值。