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Identification and fine mapping of a recessive gene controlling zebra leaf phenotype in maize
Molecular Breeding ( IF 3.1 ) Pub Date : 2021-01-22 , DOI: 10.1007/s11032-021-01202-7
Guangsheng Yuan 1 , Yucui Li 1 , Benfang Chen 1 , Hui He 1 , Zhiying Wang 1 , Jiahao Shi 1 , Yan Yang 1 , Chaoying Zou 1 , Guangtang Pan 1
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Leaf color mutant is an important resource for studying chlorophyll biosynthesis and chloroplast development in maize. Here, a novel mutant zebra crossband 9 (zb9) with transverse green-/yellow-striped leaves appeared from ten-leaf stage until senescence was identified from mutant population derived from the maize inbred line RP125. The yellow section of the zb9 mutant displays a reduction of chlorophyll and carotenoid contents, as well as impaired chloroplast structure. Genetic analysis showed that the zb9 mutant phenotype was caused by a single recessive gene. Map-based cloning demonstrated that the zb9 locus was delimited into a 648 kb region on chromosome 1 covering thirteen open reading frames (ORFs). Among them, a point mutation (G to A) in exon 2 of the gene Zm00001d029151, named Zmzb9, was identified based on sequencing analysis. The causal gene Zmzb9 encodes UDP-glucose-4-epimerase 4 (UGE4), a key enzyme involved in chloroplast development and was considered as the only candidate gene controlling the mutant phenotype. Expression patterns indicated that the causal gene was abundantly expressed in the leaves and sheaths, as well as significantly downregulated in the mutant compared to that in the wild type. Subcellular localization showed that ZmZB9 was localized in chloroplasts and implied the putative gene involved in chloroplast development. Taken together, we propose that the causal gene Zmzb9 tightly associated with the zebra leaf phenotype, and the obtained gene here will help to uncover the regulatory mechanism of pigment biosynthesis and chloroplast development in maize.



中文翻译:

控制玉米斑马叶表型的隐性基因的鉴定和精细定位

叶色突变体是研究玉米叶绿素生物合成和叶绿体发育的重要资源。在这里,从玉米自交系 RP125 衍生的突变体种群中,从十叶期开始出现一种具有横向绿/黄条纹叶的新型突变体斑马杂交带9 ( zb9 ),直到衰老为止。zb9突变体的黄色部分显示叶绿素和类胡萝卜素含量减少,以及叶绿体结构受损。遗传分析表明zb9突变表型是由单个隐性基因引起的。基于图位的克隆表明,zb9基因座被划分为 1 号染色体上的 648 kb 区域,覆盖 13 个开放阅读框 (ORF)。其中,通过测序分析,鉴定出Zm00001d029151基因2号外显子的点突变(G至A),命名为Zmzb9 。致病基因Zmzb9编码 UDP-葡萄糖-4-差向异构酶 4 (UGE4),这是一种参与叶绿体发育的关键酶,被认为是控制突变表型的唯一候选基因。表达模式表明,致病基因在叶和鞘中大量表达,并且与野生型相比,突变体中的表达显着下调。亚细胞定位表明ZmZB9定位于叶绿体中,暗示该基因参与叶绿体发育。综上所述,我们提出因果基因Zmzb9与斑马叶表型密切相关,此处获得的基因将有助于揭示玉米色素生物合成和叶绿体发育的调控机制。

更新日期:2021-01-22
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