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Performing under pressure: esterification activity of dry fermented solids in subcritical and supercritical CO2
Biotechnology Letters ( IF 2.7 ) Pub Date : 2020-10-13 , DOI: 10.1007/s10529-020-03029-2
Dominicky Cristina Serrano 1 , Marcos Lúcio Corazza 1, 2 , David Alexander Mitchell 1, 3 , Nadia Krieger 1, 4
Affiliation  

OBJECTIVE Lipases are often used in immobilized form, but commercial immobilized lipases are costly. An alternative is to produce lipases in solid-state fermentation, dry the solids and then use the "dry fermented solids" (DFS) directly. We produced DFS by growing Burkholderia contaminans on a mixture of sugarcane bagasse and sunflower seed meal and used the DFS to esterify oleic acid with ethanol in subcritical and supercritical CO2 at 40 °C. RESULTS Compared to a control without CO2 at atmospheric pressure, subcritical CO2 at 30 bar improved esterification activity 1.2-fold. Higher pressures, including supercritical pressures up to 150 bar, reduced activity to less than 80% of the control. At 30 bar, the esterification activity was improved a further 1.8-fold with the addition of 9% water (i.e. 9 g water per 100 g oleic acid) to the reaction medium. CONCLUSION A subcritical CO2 atmosphere, with the addition of a small amount of water, improved the esterification activity of DFS containing lipases of Burkholderia contaminans.

中文翻译:

在压力下进行:干燥发酵固体在亚临界和超临界 CO2 中的酯化活性

目标脂肪酶通常以固定形式使用,但商业固定化脂肪酶价格昂贵。另一种方法是在固态发酵中生产脂肪酶,干燥固体,然后直接使用“干发酵固体”(DFS)。我们通过在甘蔗渣和葵花籽粕的混合物上种植伯克霍尔德氏菌污染物来生产 DFS,并使用 DFS 在 40 °C 的亚临界和超临界 CO2 中用乙醇酯化油酸。结果 与大气压下不含 CO2 的对照相比,30 bar 下的亚临界 CO2 将酯化活性提高了 1.2 倍。更高的压力,包括高达 150 bar 的超临界压力,将活性降低到低于对照的 80%。在 30 bar 下,添加 9% 的水(即 9 g 水/100 g 油酸)到反应介质中。结论 在亚临界 CO2 气氛中,加入少量水,可提高含有伯克霍尔德菌污染脂肪酶的 DFS 的酯化活性。
更新日期:2020-10-13
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