Citrus greening disease or huanglongbing (HLB) caused by Candidatus Liberibacter asiaticus (CLas) limits citrus production worldwide. CLas is transmitted by the Asian citrus psyllid (ACP), Diaphorina citri (Hemiptera: Psyllidae) in a persistent-propagative manner. Understanding the molecular interaction between CLas and ACP and interrupting the interrelationship can provide an alternative to insecticides for managing citrus greening disease. Transcriptome analysis of ACP in response to CLas showed differential expression of 3911 genes (2196 upregulated, and 1715 downregulated) including the key genes of ACP involved in cytoskeleton synthesis and nutrition-related proteins, such as vitellogenins, extensin, laminin, tropomyosin, troponin C, and flightin. Majority of the differentially expressed genes were categorized under molecular functions followed by cellular components and biological processes. KEGG pathway analysis showed differential regulation of carbohydrate, nucleotide, and energy metabolic pathways, the endocytotic pathway, and the defense-related pathways. Differential regulation of genes associated with the key pathways might favour CLas to become systemic and propagate in its insect vector. The study provides an understanding of genes involved in circulation of CLas in ACP. The candidate genes involved in key physiological processes and CLas transmission by ACP would be potential targets for sustainable management of ACP and CLas.

由Candidatus Liberibacter asiaticus ( C Las)引起的柑橘绿化病或黄龙病 (HLB ) 限制了全球柑橘的生产。C Las 由亚洲柑橘木虱 (ACP)、柑橘木虱( Hemiptera: Psyllidae) 以持续繁殖方式传播。了解C Las 和 ACP之间的分子相互作用并中断相互关系可以为防治柑橘绿化病提供杀虫剂的替代方案。响应C的 ACP 的转录组分析Las 显示出 3911 个基因（2196 个上调，1715 个下调）的差异表达，包括参与细胞骨架合成和营养相关蛋白的 ACP 关键基因，如卵黄原蛋白、伸长蛋白、层粘连蛋白、原肌球蛋白、肌钙蛋白 C 和飞行蛋白。大多数差异表达的基因被归类为分子功能，其次是细胞成分和生物过程。KEGG 通路分析显示碳水化合物、核苷酸和能量代谢通路、内吞通路和防御相关通路的差异调节。与关键途径相关的基因的差异调节可能有利于C Las 成为系统性并在其昆虫载体中繁殖。该研究提供了对参与C循环的基因的理解拉斯在 ACP。参与关键生理过程和ACP 传递C Las 的候选基因将成为 ACP 和C Las 可持续管理的潜在目标。