The challenges with scaffold profiling of cell-based assay includes accelerated cancer cell proliferation, induced scaffold toxicity, and identifying irrelevant cancer cell-based assays in batch assessments. This study investigates profiling carcinoma of breast cancer cells of MCF-7 model systems using silica nanoparticles scaffold sourced from synthetic materials and plant extracts. Herein, the engineered tissue scaffolds were used to create temporary structures for cancer cell attachments, differentiation, and subsequently to assess the metabolic activity of the cancer cell colonies. The cell viability of the cancer cells was assessed using the tetrazolium compound (MTS reagent), which was reduced to colored formazan, to indicate metabolically active cancer cells in a proliferating assay. We aimed to develop cancer cell-based scaffolds that not only mimic the neoplastic activity, but that also allowed synergistic interaction with cisplatin for in vitro assay screening.

中文翻译：

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分析二氧化硅纳米材料衍生的单一、2 和 3 复合系统支架的肿瘤微环境

基于细胞的检测支架分析的挑战包括加速癌细胞增殖、诱导支架毒性，以及在批量评估中识别不相关的基于癌细胞的检测。本研究使用源自合成材料和植物提取物的二氧化硅纳米粒子支架，研究了 MCF-7 模型系统的乳腺癌细胞癌谱分析。在此，工程化组织支架用于为癌细胞附着、分化以及随后评估癌细胞集落的代谢活性创建临时结构。使用四唑化合物（MTS 试剂）评估癌细胞的细胞活力，该化合物被还原为有色甲臜，以在增殖测定中指示代谢活跃的癌细胞。