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Osa-miR820 regulatory node primes rice plants to tolerate salt stress in an agronomically advantageous manner
bioRxiv - Plant Biology Pub Date : 2021-01-21 , DOI: 10.1101/2021.01.20.427536
Neha Sharma , Sudhir Kumar , Neeti Sanan-Mishra

Plant microRNAs (miRs) play an important role in regulating gene expression under normal and stressful environments. Here we report the functional implications on the role of OsamiR820, which can be grouped as a young, rice specific miR. It is a member of the class II transposon derived small RNA family and is processed as 21 nt and 24 nt length variants, respectively. Size of the miR820 family varies from 1 to 16 across the Oryza AA genomes. The 21 nt Osa miR820 negatively regulates a de novo methylase, OsDRM2 (domains rearranged methyl transferase) that prevents methylation of the CACTA transposon loci in the rice genome. In an earlier report we have detailed the expression profiles of Osa miR820 and its target in abiotic stress responses using rice varieties exhibiting varying response to salt stress. In this study, artificial miR based approach was employed to specifically overexpress 21 nt Osa-miR820 in rice plants (OX 820). These plants exhibited enhanced vigour, 30% increase in the number of spikelets per panicle and increased grain filling, under normal and salt stress conditions. The OX 820 lines showed a better water use efficiency and higher proline accumulation under salt stress. These plants can serve as a useful source for dissecting the molecular machinery governed by Osa miR820:DRM2 node to prime tolerance to salt stress in an agronomically advantageous manner.

中文翻译:

Osa-miR820调节节点以农艺上的优势引发水稻植株耐盐胁迫

在正常和压力环境下,植物微RNA(miR)在调节基因表达中起着重要作用。在这里,我们报告了对OsamiR820的作用的功能含义,可以将其归类为年轻的水稻特异性miR。它是II类转座子衍生的小RNA家族的成员,分别被加工为21 nt和24 nt长度的变体。在整个Oryza AA基因组中,miR820家族的大小从1到16不等。21 nt Osa miR820负调控从头甲基化酶OsDRM2(结构域重排的甲基转移酶),可防止水稻基因组中CACTA转座子基因座的甲基化。在较早的报告中,我们使用了对盐胁迫表现出不同反应的水稻品种,详细描述了Osa miR820及其目标在非生物胁迫响应中的表达谱。在这个研究中,基于人工miR的方法被用于在水稻植物中特别过量表达21 nt Osa-miR820(OX 820)。在正常和盐胁迫条件下,这些植物表现出增强的活力,每穗小穗数增加30%,谷物填充增加。OX 820品系在盐胁迫下表现出更好的水分利用效率和更高的脯氨酸积累量。这些植物可以作为有用的资料来源,剖析由Osa miR820:DRM2节点控制的分子机制,以农艺上有利的方式引发对盐胁迫的耐受性。OX 820品系在盐胁迫下表现出更好的水分利用效率和更高的脯氨酸积累量。这些植物可以作为有用的资料来源,剖析由Osa miR820:DRM2节点控制的分子机制,以农艺上有利的方式引发对盐胁迫的耐受性。OX 820品系在盐胁迫下表现出更好的水分利用效率和更高的脯氨酸积累量。这些植物可以作为有用的资料来源,剖析由Osa miR820:DRM2节点控制的分子机制,以农艺上有利的方式引发对盐胁迫的耐受性。
更新日期:2021-01-21
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