$-1$ programmed ribosomal frameshifting ($-1\mathrm{PRF}$) is stimulated by structures in messenger RNA (mRNA), but the factors determining $-1\mathrm{PRF}$ efficiency are unclear. We show that $-1\mathrm{PRF}$ efficiency varies directly with the conformational heterogeneity of the stimulatory structure, quantified as the Shannon entropy of the state occupancy, for a panel of stimulatory structures with efficiencies from 2% to 80%. The correlation is force dependent and vanishes at forces above those applied by the ribosome. These results support the hypothesis that heterogeneous conformational dynamics are a key factor in stimulating $-1\mathrm{PRF}$.

中文翻译：

---

力光谱测量的mRNA结构的构象香农熵预测−1程控的核糖体移码刺激的效率

$-\mathrm{1个}$ 程序性核糖体移码（$-\mathrm{1个}\mathrm{PRF}$）由信使RNA（mRNA）中的结构刺激，但决定因素 $-\mathrm{1个}\mathrm{PRF}$效率尚不清楚。我们证明$-\mathrm{1个}\mathrm{PRF}$对于一组效率为2％至80％的刺激结构，效率直接取决于刺激结构的构象异质性（量化为状态占用的香农熵）。相关性是受力依赖的，并且在高于核糖体所施加的力时消失。这些结果支持以下假设：异构构象动力学是刺激的关键因素。$-\mathrm{1个}\mathrm{PRF}$。