Malassezia species are a major part of the normal mycobiota and colonize mainly sebum-rich skin regions of the body. This group of fungi cause a variety of infections such as pityriasis versicolor, folliculitis, and fungaemia. In particular, Malassezia sympodialis and its allergens have been associated with non-infective inflammatory diseases such as seborrheic dermatitis and atopic eczema. The aim of this study was to investigate the host response to M. sympodialis on oily skin (supplemented with oleic acid) and non-oily skin using an ex vivo human skin model. Host-pathogen interactions were analyzed by SEM, histology, gene expression, immunoassays and dual species proteomics. The skin response to M. sympodialis was characterized by increased expression of the genes encoding β-defensin 3 and RNase7, and by high levels of S100 proteins in tissue. Supplementation of oleic acid onto skin was associated with direct contact of yeasts with keratinocytes and epidermal damage. In oily conditions, there was increased expression of IL18 but no expression of antimicrobial peptide genes in the skin’s response to M. sympodialis. In supernatants from inoculated skin plus oleic acid, TNFα, IL-6, and IL1-β levels were decreased and IL-18 levels were significantly increased.

马拉色菌属这些物种是正常分枝杆菌的主要部分，主要定植在人体皮脂丰富的皮肤区域。这组真菌引起多种感染，如杂色性糠疹，毛囊炎和真菌病。特别是，拟南芥其变应原与非感染性炎症疾病如脂溢性皮炎和特应性湿疹有关。这项研究的目的是调查宿主对交趾分枝杆菌 在油性皮肤上（补充油酸）和非油性皮肤上使用 离体人体皮肤模型。通过SEM，组织学，基因表达，免疫测定和双物种蛋白质组学分析宿主-病原体的相互作用。皮肤反应交趾分枝杆菌其特征是编码β-防御素3和RNase7的基因表达增加，以及组织中S100蛋白水平高。在皮肤上补充油酸与酵母与角质形成细胞的直接接触和表皮损伤有关。在油性条件下，表达增加白介素18 但在皮肤对皮肤的反应中没有抗菌肽基因的表达 交趾分枝杆菌。在接种皮肤加油酸的上清液中，TNFα，IL-6和IL1-β水平降低，IL-18水平显着升高。