Acmella oleracea L. is an important medicinal plant, commonly known as the toothache plant. It is a rich source of secondary metabolites used for the treatment of different human disorders. The demand for Acmella oleracea L. has increased due to its putative health benefits (in terms of both biomass quantity and bioactive compound purification). In vitro plant cultures have allowed the rapid increase of raw material availability through the use of suitable regeneration and multiplication systems. On the other hand, there is a general lack of methods for Acmella genetic transformation as a promising new technological approach for the improvement of secondary metabolites. In this work, an efficient transformation protocol has been established using the Agrobacterium tumefaciens LBA4404 strain bearing the binary vector pBI121 containing the NPTII gene for the resistance to kanamycin. Plant genetic transformation has been verified by direct polymerase chain reaction and GUS assay on regenerants. Transformation efficiency has been affected by the high level of the selection agent kanamycin. To our knowledge, this is the first report on the genetic transformation of A. oleracea, paving the way to further studies to improve in vitro plant growth and secondary metabolite production.

中文翻译：

---

用根癌农杆菌遗传转化油菜菌的一种有效方法（Spilanthes acmella Linn。）

菜豆（Acmella oleracea L.）是一种重要的药用植物，通常被称为牙痛植物。它是用于治疗不同人类疾病的次级代谢产物的丰富来源。由于假定的健康益处（就生物量和生物活性化合物的纯化而言），对油菜无花果的需求增加了。体外植物培养可以通过使用合适的再生和繁殖系统来快速提高原料利用率。另一方面，普遍缺乏作为改良次生代谢物的有前途的新技术方法的Acmella基因转化方法。在这项工作中，使用带有包含对卡那霉素具有抗性的NPTII基因的二元载体pBI121的根癌农杆菌LBA4404菌株。植物遗传转化已通过直接聚合酶链式反应和再生剂的GUS分析得到验证。高水平的选择剂卡那霉素影响了转化效率。据我们所知，这是关于油茶遗传转化的第一份报告，为进一步研究改善体外植物生长和次生代谢产物的生产铺平了道路。