ABSTRACT

Sasa quelpaertensis Nakai extract (SQE) has long been used as a traditional medicine and health drink. The present study aims to examine the properties and antioxidant activity of the phytochemicals isolated from S. quelpaertensis. Therefore, the SQE was fractionated with n-hexane, chloroform (CHCl₃), ethyl acetate (EtOAc), and n-butanol (BuOH). Phytochemicals present in the solvent fraction were also isolated using a high-performance liquid chromatography (HPLC) system. In addition, their DPPH radical scavenging ability and NO production inhibitory effect were compared. The results showed that phytochemicals p-hydroxybenzaldehyde (1), salicylic acid (2), syringaldehyde (3), methyl cis-p-hydroxycinnamate (4), methyl trans-p-hydroxycinnamate (5), p-coumaric acid (6), 2,3-dihydrozypropyl 9Z,12Z-octadecadienoate (7), (+)-(6S,7aS)-epilolide (8), (-)-(6 R,7aS)-loliolide (9), naringenin (10), 3-O-p-coumaroyl-1-(4-hydroxy-3,5-dimethoxyphenyl)-1-O-β-glucopyranosylpropanol (11), tricin (12), and tricin 7-O-b-D-glucopyranoside (13) were isolated from CHCl₃, EtOAc, and BuOH fractions and then identified using nuclear magnetic resonance (NMR) spectrometry. This is an initial study on compounds 2, 3, 4, 5, 7, 8, and 9 obtained from S. quelpaertensis. Compounds 11 (IC₅₀ 120.3 μM) and 7 (IC₅₀ 43.62 μM) showed significant DPPH radical scavenging activity and anti-inflammatory activity, respectively. We believe these results may provide the basic data for developing effective antioxidants using the SQE.

摘要

Sasa quelpaertensis Nakai提取物（SQE）长期用作传统药物和健康饮料。本研究的目的是检查从奎尔帕特酵母中分离出的植物化学物质的性质和抗氧化活性。因此，将SQE用正己烷，氯仿（CHCl ₃），乙酸乙酯（EtOAc）和正丁醇（BuOH）分馏。还使用高效液相色谱（HPLC）系统分离了存在于溶剂馏分中的植物化学物质。另外，比较了它们的DPPH自由基清除能力和NO产生抑制作用。结果表明，植物化学物质对羟基苯甲醛（1），水杨酸（2），丁香醛（3），顺式对羟基肉桂酸甲酯（4），甲基反式对羟基肉桂酸酯（5），对香豆酸（6），2,3-二氢丙基丙基9 Z，12 Z-十八碳二烯酸酯（7），（+）-（6 S，7a S）-上环内酯（8）， （-）-（6 R，7aS）-卤代内酯（9），柚皮苷（10），3- Op-香豆酰基-1-（4-羟基-3,5-二甲氧基苯基）-1- O-β-吡喃葡萄糖基丙醇（11从CHCl ₃，EtOAc和BuOH馏分中分离出Tricin （12）和Tricin 7- O -bD-吡喃葡萄糖苷（13），然后使用核磁共振（NMR）光谱进行鉴定。这是对从奎尔伯特酵母获得的化合物2、3、4、5、7、8和9的初步研究。化合物11（IC ₅₀120.3μM）和7（IC ₅₀ 43.62μM）分别显示出显着的DPPH自由基清除活性和抗炎活性。我们相信这些结果可能为使用SQE开发有效的抗氧化剂提供基础数据。