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High-capacity multimodal anion-exchange membranes for polishing of therapeutic proteins
Biotechnology Progress ( IF 2.9 ) Pub Date : 2021-01-21 , DOI: 10.1002/btpr.3129
Joshua Osuofa 1 , Daniel Henn 2 , Jinxiang Zhou 2 , Anna Forsyth 2 , Scott M Husson 1
Affiliation  

This contribution reports on a study using Purexa™-MQ multimodal anion-exchange (AEX) membranes for protein polishing at elevated solution conductivities. Dynamic binding capacities (DBC10) of bovine serum albumin (BSA), human immunoglobulins, and salmon sperm DNA (ss-DNA) are reported for various salt types, salt concentrations, flowrates, and pH. Using 1 mg/ml BSA, DBC10 values for Purexa™-MQ were >90 mg/ml at conductivities up to 15 mS/cm. The membranes maintained a high, salt-tolerant BSA DBC10 of 89.8 ± 2.7 (SD) over the course of 100 bind-elute cycles. Polishing studies with acidic and basic monoclonal antibodies at >2 kg/L loads showed that Purexa™-MQ had higher clearance of host cell proteins and aggregate species at high conductivity (13 mS/cm) and in the presence of phosphate than other commercial AEX media. Purexa™-MQ also had a high ss-DNA DBC10 of 50 mg/ml at conductivities up to 15 mS/cm, markedly outperforming other commercial products. In addition to the effectiveness of Purexa™-MQ for protein polishing at elevated solution conductivities, its unusually high binding capacity for ss-DNA indicates potential applications for plasmid DNA purification.

中文翻译:

用于治疗性蛋白质抛光的高容量多峰阴离子交换膜

该贡献报告了一项使用 Purexa™-MQ 多峰阴离子交换 (AEX) 膜在提高溶液电导率下进行蛋白质抛光的研究。报告了牛血清白蛋白 (BSA)、人免疫球蛋白和鲑鱼精子 DNA (ss-DNA) 的动态结合能力 (DBC 10 ) 用于各种盐类型、盐浓度、流速和 pH 值。使用 1 mg/ml BSA,Purexa™-MQ 的 DBC 10值 >90 mg/ml,电导率高达 15 mS/cm。膜保持高耐盐 BSA DBC 10在 100 个结合洗脱循环过程中为 89.8 ± 2.7 (SD)。使用 >2 kg/L 负载的酸性和碱性单克隆抗体进行抛光研究表明,与其他商业 AEX 相比,Purexa™-MQ 在高电导率 (13 mS/cm) 和磷酸盐存在下对宿主细胞蛋白和聚集物的清除率更高媒体。Purexa™-MQ在电导率高达 15 mS/cm 时还具有 50 mg/ml的高 ss-DNA DBC 10 ,明显优于其他商业产品。除了 Purexa™-MQ 在提高溶液电导率下对蛋白质精制的有效性外,其对 ss-DNA 的异常高结合能力表明了质粒 DNA 纯化的潜在应用。
更新日期:2021-01-21
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