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Hsa_circ_0001073 targets miR ‐626/ LIFR axis to inhibit lung cancer progression
Environmental Toxicology ( IF 4.5 ) Pub Date : 2021-01-21 , DOI: 10.1002/tox.23104
Qun Liu 1 , Gang Cao 2 , Yufeng Wan 3 , Chuanqin Xu 3 , Yuanqiang He 3 , Gang Li 3
Affiliation  

Circular RNAs (circRNAs) are associated with lung cancer progression. However, it is unclear whether and how circRNA hsa_circ_0001073 (circ_0001073) are involved in lung cancer progression. circ_0001073, microRNA (miR)-626, and leukemia inhibitory factor receptor (LIFR) abundances were determined via quantitative reverse transcription polymerase chain reaction or western blot. Cell viability, invasion, and apoptosis were analyzed by cell counting kit-8, transwell analysis and flow cytometry, respectively. The target correlation was tested by dual-luciferase reporter analysis or RNA immunoprecipitation. Results showed that circ_0001073 abundance was down-regulated in lung cancer cells. circ_0001073 constrained cell viability and invasion and facilitated apoptosis in lung cancer cells. miR-626 was targeted via circ_0001073, and circ_0001073 inhibited lung cancer progression via reducing miR-626 expression. LIFR was targeted via miR-626, and miR-626 knockdown constrained cell viability and invasion and facilitated apoptosis in lung cancer cells via up-regulating LIFR. circ_0001073 increased LIFR expression via miR-626 in lung cancer cells. In conclusion, circ_0001073 represses lung cancer progression via miR-626/LIFR axis, indicating the potential value of circ_0001073 in lung cancer treatment.

中文翻译:

Hsa_circ_0001073靶向miR-626/LIFR轴抑制肺癌进展

环状RNA(circRNA)与肺癌进展相关。然而,circRNA hsa_circ_0001073(circ_0001073)是否以及如何参与肺癌进展尚不清楚。circ_0001073、microRNA (miR)-626 和白血病抑制因子受体 (LIFR) 的丰度是通过定量逆转录聚合酶链反应或蛋白质印迹确定的。分别通过细胞计数试剂盒-8、transwell 分析和流式细胞术分析细胞活力、侵袭和凋亡。通过双荧光素酶报告基因分析或 RNA 免疫沉淀测试目标相关性。结果显示circ_0001073丰度在肺癌细胞中被下调。circ_0001073 限制肺癌细胞的细胞活力和侵袭并促进细胞凋亡。miR-626 通过 circ_0001073 靶向,circ_0001073 通过降低 miR-626 表达抑制肺癌进展。LIFR 通过 miR-626 靶向,miR-626 敲低限制了细胞活力和侵袭,并通过上调 LIFR 促进了肺癌细胞的凋亡。circ_0001073 通过 miR-626 在肺癌细胞中增加 LIFR 表达。总之,circ_0001073通过miR-626/LIFR轴抑制肺癌进展,表明circ_0001073在肺癌治疗中的潜在价值。
更新日期:2021-01-21
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