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Investigation of the Physiological, Biochemical and Antifungal Susceptibility Properties of Candida auris
Mycopathologia ( IF 5.5 ) Pub Date : 2021-01-21 , DOI: 10.1007/s11046-020-00526-w
Mengqian Du 1, 2 , Weimin Hu 1, 3, 4 , Takashi Tamura 1 , Mohamed Mahdi Alshahni 5, 6 , Kazuo Satoh 1, 5 , Chiaki Yamanishi 1 , Toshio Naito 2 , Koichi Makimura 1, 5, 6
Affiliation  

Background

Candida auris is an emerging pathogen associated with outbreaks in clinical settings. Isolates of the pathogen have been geographically clustered into four clades with high intra-clade clonality. Pathogenicity varies among the clades, highlighting the importance of understanding these differences.

Objectives

To examine the physiological and biochemical properties of each clade of C. auris to improve our understanding of the fungus.

Methods

Optimal growth temperatures of four strains from three clades, East Asia, South Asia and South Africa, were explored. Moreover, assimilation and antifungal susceptibility properties of 22 C. auris strains from the three clades were studied.

Results

The optimal growth temperatures of all strains were 35–37 °C. Assimilation testing demonstrated that the commercial API ID 32 C system can be used to reliably identify C. auris based on the biochemical properties of the yeast. Notably, C. auris can be uniquely differentiated from commonly clinical fungi by its ability to assimilate raffinose and inability to utilize D-xylose, suggesting a useful simple screening tool. The antifungal susceptibility results revealed that all strains are resistant against fluconazole (minimal inhibitory concentration (MIC) 4 to > 64 µg/mL) and miconazole (MIC 8 to > 16 µg/mL), with strains from the Japanese lineage showing relatively lower MIC values (1–4 µg/mL). Conversely, itraconazole, voriconazole, amphotericin B, micafungin and caspofungin were active against most of the tested strains. On the clade level, East Asian strains generally showed lower MICs against azoles comparing to the other clades, while they displayed MICs against flucytosine higher than those of strains from South Africa and South Asia clades.

Conclusion

Our data suggest a simple identification approach of C. auris based on its physiological and biochemical properties and highlight aspects of C. auris population from various clades.



中文翻译:

耳念珠菌生理、生化和抗真菌药敏特性的研究

背景

耳念珠菌是一种与临床环境中爆发相关的新兴病原体。病原体的分离株在地理上聚集成四个进化枝,具有高进化枝内克隆性。致病性因进化枝而异,突出了了解这些差异的重要性。

目标

检查每个C. auris进化枝的生理和生化特性,以提高我们对真菌的了解。

方法

探索了来自东亚、南亚和南非三个进化枝的四种菌株的最佳生长温度。此外,研究了来自三个进化枝的 22 C. auris菌株的同化和抗真菌敏感性特性。

结果

所有菌株的最佳生长温度为 35-37°C。同化测试表明,商业 API ID 32 C 系统可用于根据酵母的生化特性可靠地识别耳念珠菌。值得注意的是,C. auris可以通过同化棉子糖的能力和不能利用 D-木糖的能力与常见的临床真菌进行独特的区分,这表明它是一种有用的简单筛选工具。抗真菌药敏结果显示,所有菌株对氟康唑(最低抑菌浓度 (MIC) 4 至 > 64 µg/mL)和咪康唑(MIC 8 至 > 16 µg/mL)均具有抗性,日本谱系的菌株显示出相对较低的 MIC值 (1–4 µg/mL)。相反,伊曲康唑、伏立康​​唑、两性霉素 B、米卡芬净和卡泊芬净对大多数受试菌株均有活性。在进化枝水平上,与其他进化枝相比,东亚菌株通常对唑类显示出较低的 MIC,而它们对氟胞嘧啶的 MIC 高于来自南非和南亚进化枝的菌株。

结论

我们的数据提出了一种简单的C. auris识别方法,该方法基于其生理和生化特性,并突出了来自不同进化枝的C. auris种群的各个方面。

更新日期:2021-01-21
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