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Morpho-taxometrical and molecular characterization of Steinernema abbasi (Nematoda: Steinernematidae) and its pathogenicity and generative potential against lepidopteran pests
Egyptian Journal of Biological Pest Control ( IF 2.4 ) Pub Date : 2021-01-20 , DOI: 10.1186/s41938-020-00359-1
Heena , Aasha Rana , Aashaq Hussain Bhat , Ashok Kumar Chaubey

An entomopathogenic nematode (EPN) was recovered by using Galleria baiting technique from the soils of marigold fields of Noida, Uttar Pradesh, India. Based on morphological, morphometrical and molecular characterizations, the isolated strain was identified as Steinernema abbasi and tagged as CS38. The isolated strain was conspecific to original description with minor deviations. Infective juveniles (IJs) of present strain were longer than original description. Molecular analysis was done using ITS1-5.8S-ITS2 and D2D3 regions. Pathogenicity and generative potential of the present strain CS38 were tested against larvae of 3 lepidopteran insect pests, namely, Galleria mellonella L., Helicoverpa armigera (Hb.) and Spodoptera litura (Fab.). Different concentrations of IJs/larva, viz. 25, 50, 100, and 200 IJs/larva, were used for bioassay trails. All experiments were repeated thrice to reach the optimum authenticity. Results of bioassays revealed that isolate CS38 was highly virulent against the 3 insect pests and caused (100%) mortality within 48 h under laboratory conditions. Generative potential of the studied S. abbasi CS38 was recorded high in G. mellonella (19 × 104 IJs/larva) at 100 IJs/larva concentration, followed by H. armigera (63.4 × 103 IJs/larva) and S. litura (60.5 × 103 IJs/larva). Isolate CS38 is an indigenous, dominant and highly virulent strain that can be utilized as a biological control agent against the three studied insects. Moreover, it can be used for commercialization of the production of EPN-based biopesticide to be added under Integrated Pest Management in Indian agriculture system.

中文翻译:

拟南芥Steinernema abbasi(Nematoda:Steinernematidae)的形态形态学和分子特征及其对鳞翅目害虫的致病性和产生潜力

使用Galleria诱饵技术从印度北方邦诺伊达的万寿菊土壤中回收了昆虫病原线虫(EPN)。根据形态,形态和分子特征,分离出的菌株被鉴定为Steinernema abbasi,标记为CS38。分离出的菌株与原始描述是特定的,只有很小的偏差。当前菌株的感染性幼体(IJs)比原始描述的更长。使用ITS1-5.8S-ITS2和D2D3区进行分子分析。测试了本菌株CS38菌株对3种鳞翅目昆虫害虫的幼虫的致病性和产生潜力,所述害虫是Malleria mellonella L.,棉铃虫(Helicoverpa armigera)(Hb。)和斜纹夜蛾(Spodoptera litura)(Fab。)。不同浓度的IJ /幼虫,即。25、50、100和200 IJ /幼虫用于生物分析。重复所有实验三次以达到最佳真实性。生物测定的结果表明,分离物CS38对3种害虫具有高度毒性,并在实验室条件下48小时内引起(100%)死亡率。所研究的S. abbasi CS38的生殖潜能在幼虫浓度为100 IJs /幼虫的G. mellonella(19×104 IJs /幼虫)中较高,其次是棉铃虫H. armigera(63.4×103 IJs /幼虫)和S. litura(60.5)。 ×103 IJ /幼虫)。分离株CS38是一种本地的,显性的和高毒力的菌株,可以用作针对三种研究昆虫的生物防治剂。而且,它可以用于基于EPN的生物农药生产的商业化,并将其添加到印度农业系统的病虫害综合治理中。生物测定的结果表明,分离物CS38对3种害虫具有高度毒性,并在实验室条件下48小时内引起(100%)死亡率。所研究的S. abbasi CS38的生殖潜能在幼虫浓度为100 IJs /幼虫的G. mellonella(19×104 IJs /幼虫)中较高,其次是棉铃虫H. armigera(63.4×103 IJs /幼虫)和S. litura(60.5)。 ×103 IJ /幼虫)。分离株CS38是一种本地的,显性的和高毒力的菌株,可以用作针对三种研究昆虫的生物防治剂。而且,它可以用于基于EPN的生物农药生产的商业化,并将其添加到印度农业系统的病虫害综合治理中。生物测定的结果表明,分离物CS38对3种害虫具有高度毒性,并在实验室条件下48小时内引起(100%)死亡率。所研究的S. abbasi CS38的生殖潜能在幼虫浓度为100 IJs /幼虫的G. mellonella(19×104 IJs /幼虫)中较高,其次是棉铃虫H. armigera(63.4×103 IJs /幼虫)和S. litura(60.5)。 ×103 IJ /幼虫)。分离株CS38是一种本地的,显性的和高毒力的菌株,可以用作针对三种研究昆虫的生物防治剂。而且,它可以用于基于EPN的生物农药生产的商业化,并将其添加到印度农业系统的病虫害综合治理中。所研究的S. abbasi CS38的生殖潜能在幼虫浓度为100 IJs /幼虫的G. mellonella(19×104 IJs /幼虫)中较高,其次是棉铃虫H. armigera(63.4×103 IJs /幼虫)和S. litura(60.5)。 ×103 IJ /幼虫)。分离株CS38是一种本地的,显性的和高毒力的菌株,可以用作针对三种研究昆虫的生物防治剂。而且,它可以用于基于EPN的生物农药生产的商业化,并将其添加到印度农业系统的病虫害综合治理中。所研究的S. abbasi CS38的生殖潜能在幼虫浓度为100 IJs /幼虫的G. mellonella(19×104 IJs /幼虫)中较高,其次是棉铃虫H. armigera(63.4×103 IJs /幼虫)和S. litura(60.5)。 ×103 IJ /幼虫)。分离株CS38是一种本地的,显性的和高毒力的菌株,可以用作针对三种研究昆虫的生物防治剂。而且,它可以用于基于EPN的生物农药生产的商业化,并将其添加到印度农业系统的病虫害综合治理中。优势和高毒力菌株,可用作对三种研究昆虫的生物防治剂。而且,它可以用于基于EPN的生物农药生产的商业化,并将其添加到印度农业系统的病虫害综合治理中。优势和高毒力菌株,可用作对三种研究昆虫的生物防治剂。而且,它可以用于基于EPN的生物农药生产的商业化,并将其添加到印度农业系统的病虫害综合治理中。
更新日期:2021-01-20
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