Thioflavin-T is used to image amyloid aggregates because of the excellent turn-on fluorescence properties, but binding affinities are low. By mounting multiple dye units on the surface of a vesicle, the binding affinity for α-synuclein fibrils is increased by three orders of magnitude, and the optical response is increased. Cooperative interactions of the dye headgroup and lipid with the protein provide a general strategy for the construction of multivalent amyloid probes based on vesicles.

中文翻译：

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淀粉样蛋白聚集体的脂质体增强检测

由于具有出色的开启荧光特性，硫磺素-T 可用于对淀粉样蛋白聚集体进行成像，但结合亲和力较低。通过在囊泡表面安装多个染料单元，对 α-突触核蛋白原纤维的结合亲和力增加了三个数量级，光学响应也增加了。染料头部基团和脂质与蛋白质的协同相互作用为构建基于囊泡的多价淀粉样蛋白探针提供了一般策略。