Rapid and cost-effective in vivo assays to screen potential environmental neurodevelopmental toxicants are necessary to address the limitations of in vitro platforms, such as the inability to fully recapitulate the developmental and physiological processes of whole organisms. In the present study, a rapid zebrafish behavioral profiling assay was developed to characterize the neurodevelopmental effects of environmental substances by quantitatively evaluating multiple spontaneous movement features of zebrafish embryos. This video analysis-based assay automatically segmented every embryo and thus was able to accurately quantify spontaneous movement features, including frequency, duration, intensity, interval, and the number of continuous movements. When tested with eight environmental substances known to be neurodevelopmental toxicants, such as chlorpyrifos and bisphenol A, the assay successfully captured frequency alterations that were well-documented in previous studies while also providing additional information. Using an optimized procedure, we further assessed 132 potential neurotoxins that spanned a wide range of molecular targets, many of which were previously detected in environmental waterbodies. The distinct altered behavioral barcodes indicated that the spontaneous movement was impacted by diverse neuroactive substances, and the effects could be effectively evaluated with the developed assay. The web-based tool, named EMAnalysis, is further provided at http://www.envh.sjtu.edu.cn/zebrafish_contraction.jsp. Thus, this assay provides an efficient platform to accelerate the pace of neurotoxic environmental contaminant discoveries.

中文翻译：

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快速的斑马鱼行为分析法可加快环境神经发育毒物的鉴定

快速且经济有效的体内分析方法以筛选潜在的环境神经发育毒物对于解决体外方法的局限性是必要的平台，例如无法完全概括整个生物体的发育和生理过程。在本研究中，开发了一种快速的斑马鱼行为特征分析法，通过定量评估斑马鱼胚胎的多个自发运动特征来表征环境物质的神经发育作用。这种基于视频分析的检测方法可以自动分割每个胚胎，从而能够准确地量化自发运动特征，包括频率，持续时间，强度，间隔和连续运动次数。当用八种已知是神经发育毒物的环境物质（例如毒死rif和双酚A）进行测试时，该测定法成功捕获了先前研究中充分记录的频率变化，同时还提供了其他信息。使用优化的程序，我们进一步评估了132种潜在的神经毒素，它们跨越了广泛的分子靶标，其中许多以前是在环境水体中检测到的。行为条形码的明显变化表明，自发运动受到多种神经活性物质的影响，并且可以通过开发的检测方法有效地评估其作用。http://www.envh.sjtu.edu.cn/zebrafish_contraction.jsp上提供了名为EMAnalysis的基于Web的工具。因此，该测定提供了加速神经毒性环境污染物发现步伐的有效平台。我们进一步评估了132种潜在的神经毒素，它们涵盖了广泛的分子靶标，其中许多以前是在环境水体中检测到的。行为条形码的明显变化表明，自发运动受到多种神经活性物质的影响，并且可以通过开发的检测方法有效地评估其作用。http://www.envh.sjtu.edu.cn/zebrafish_contraction.jsp上提供了名为EMAnalysis的基于Web的工具。因此，该测定提供了加速神经毒性环境污染物发现步伐的有效平台。我们进一步评估了132种潜在的神经毒素，它们涵盖了广泛的分子靶标，其中许多以前是在环境水体中检测到的。行为条形码的明显变化表明，自发运动受到多种神经活性物质的影响，并且可以通过开发的检测方法有效地评估其作用。http://www.envh.sjtu.edu.cn/zebrafish_contraction.jsp上提供了名为EMAnalysis的基于Web的工具。因此，该测定提供了加速神经毒性环境污染物发现步伐的有效平台。使用开发的检测方法可以有效地评估效果。http://www.envh.sjtu.edu.cn/zebrafish_contraction.jsp上提供了名为EMAnalysis的基于Web的工具。因此，该测定提供了加速神经毒性环境污染物发现步伐的有效平台。使用开发的检测方法可以有效地评估效果。http://www.envh.sjtu.edu.cn/zebrafish_contraction.jsp上提供了名为EMAnalysis的基于Web的工具。因此，该测定提供了加速神经毒性环境污染物发现步伐的有效平台。