Horsegram [Macrotyloma uniflorum (Lam.) Verdc.] is an underutilized warm season diploid legume (2n=20, 22), It is consumed as a food legume in India, and animal feed and fodder in Africa and Australia. Because of its ability to grow under water-deficient and marginal soil conditions, horsegram is a preferred choice in the era of the global climatic change. In recognition of its potential as a crop species, we generated and analyzed a draft genome sequence for HPK-4. The genome sequences of HPK-4 were generated by Illumina platform. Ten chromosome-scale pseudomolecules were created by aligning scaffold sequences onto a linkage map. The total length of the ten pseudomolecules were 259.2 Mb, covering 89% of the total length of the assembled sequences. A total of 36,105 genes were predicted on the assembled sequences, and 14,736 were considered to be horsegram specific genes by comparative analysis with Phaseolus. vulgaris, Vigna. angularis, Lotus. japonicus and Arabidopsis. thaliana. The results of macrosynteny analysis suggested that the genome structure of V. angularis is more similar to horsegram than that of P. vulgaris. Diversity analysis in the 91 accessions of horsegram with dd-RAD-Seq analysis indicated narrow genetic diversity among the horsegram accessions. This is the first attempt to generate a draft genome sequence in horsegram and will provide a reference for sequence-based analysis of the horsegram germplasm to elucidate the genetic basis of important traits.

中文翻译：

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马gram（Macrotyloma uniflorum）的染色体规模的基因组草图序列。

Horsegram [硬皮豆（Lam。）Verdc。]是一种未得到充分利用的暖季二倍体豆科植物（2n = 20，22），在印度以豆类食品为食，在非洲和澳大利亚以动物饲料和饲料为食。由于其在缺水和边缘土壤条件下的生长能力，在全球气候变化时代，马斯克是首选。为了认识到其作为农作物物种的潜力，我们生成并分析了HPK-4的基因组序列草案。HPK-4的基因组序列由Illumina平台产生。通过将支架序列比对到连锁图谱上，创建了十个染色体规模的假分子。十个假分子的总长度为259.2 Mb，占组装序列总长度的89％。在组装的序列上总共预测了36105个基因，其中14个基因菜豆。寻常，豇豆。角突，莲花。刺参和拟南芥。拟南芥。macrosynteny分析的结果表明，基因组结构V.角突更类似于horsegram比的夏枯草。用dd-RAD-Seq分析对91份马文的种质进行的多样性分析表明，马文种之间的遗传多样性较窄。这是在马图中生成基因组序列草案的首次尝试，将为对马图种质进行基于序列的分析以阐明重要性状的遗传基础提供参考。