Pin nematodes (<i>Paratylenchus</i> spp.) are polyphagous parasitic species with a wide host range and geographical distribution; their diversity is unknown in the potato growing region of Alberta, Canada. The present study aims to provide morphological and molecular characterization of three pin nematode species, namely <i>P. neoprojectus</i>, <i>P. tateae</i>, and a new species, <i>Paratylenchus enigmaticus</i> sp. nov. All of them were recovered from the potato growing region of southern Alberta. The nematodes were isolated using the sieving and flotation-centrifugation method, and their morphology was assessed by light microscopy. Molecular characterization was performed using partial 18S, D2–D3 expansion domains of the 28S and ITS ribosomal genes. This study is the first report of molecular characterization of <i>P. tateae</i> and <i>P. neoprojectus</i>, being new records from southern Alberta, and two Spanish populations of <i>P. tateae</i> comprising the first report of this species in Europe. The phylogenetic analysis of the 18S, D2–D3 expansion domains of the 28S and ITS ribosomal DNA regions underscores the importance of using molecular data for accurate species identification and clarifies the status of <i>P. nanus</i> type B and <i>P. sheri</i>. Moreover, our findings will be useful to determine the impact of pin nematodes on potato production in future field research.

中文翻译：

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来自加拿大艾伯塔省南部马铃薯种植区的针状线虫（Paratylenchus spp。）的物种多样性

针状线虫（<i>副线虫</ i> spp。）是一种多食性寄生虫，具有广泛的寄主范围和地理分布。在加拿大艾伯塔省马铃薯种植区，它们的多样性是未知的。本研究旨在提供三种针状线虫物种的形态学和分子特征，即<i> P. neoprojectus </ i>，<i> Tateae </ i>和一个新的物种<i> Paratylenchus enigmaticus</ i> sp。十一月 所有这些都是从艾伯塔省南部的马铃薯种植区中回收的。使用筛分和浮选-离心法分离线虫，并通过光学显微镜评估其形态。使用28S和ITS核糖体基因的部分18S，D2-D3扩展域进行分子表征。这项研究是关于<i> tateae </ i>和<i> P.neoprojectus </ i>的分子表征的第一份报告，这是来自艾伯塔省南部的新记录，以及两个西班牙<i> Tateae种群</ i>包括该物种在欧洲的首次报道。对28S和ITS核糖体DNA区的18S，D2-D3扩展域的系统发育分析强调了使用分子数据进行准确物种识别的重要性，并阐明了<i> nanus </ i> B型和i> P. sheri </ i>。此外，我们的发现对于将来在田间研究中确定针状线虫对马铃薯生产的影响将是有用的。