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The Arabidopsis MADS-Domain Transcription Factor SEEDSTICK Controls Seed Size via Direct Activation of E2Fa
Plants ( IF 4.658 ) Pub Date : 2021-01-20 , DOI: 10.3390/plants10020192
Dario Paolo , Lisa Rotasperti , Arp Schnittger , Simona Masiero , Lucia Colombo , Chiara Mizzotti

Seed size is the result of complex molecular networks controlling the development of the seed coat (of maternal origin) and the two fertilization products, the embryo and the endosperm. In this study we characterized the role of Arabidopsis thaliana MADS-domain transcription factor SEEDSTICK (STK) in seed size control. STK is known to regulate the differentiation of the seed coat as well as the structural and mechanical properties of cell walls in developing seeds. In particular, we further characterized stk mutant seeds. Genetic evidence (reciprocal crosses) of the inheritance of the small-seed phenotype, together with the provided analysis of cell division activity (flow cytometry), demonstrate that STK acts in the earlier phases of seed development as a maternal activator of growth. Moreover, we describe a molecular mechanism underlying this activity by reporting how STK positively regulates cell cycle progression via directly activating the expression of E2Fa, a key regulator of the cell cycle. Altogether, our results unveil a new genetic network active in the maternal control of seed size in Arabidopsis.

中文翻译:

拟南芥MADS域转录因子SEEDSTICK通过直接激活E2Fa控制种子大小

种子的大小是复杂的分子网络的结果,这些分子网络控制了种皮(产于母体)和两种受精产品(胚胎和胚乳)的发育。在这项研究中,我们表征了拟南芥MADS结构域转录因子SEEDSTICK(STK)在种子大小控制中的作用。已知STK可调节种皮的分化以及发育中的种子中细胞壁的结构和机械性能。特别是,我们进一步确定了stk突变种子。小种子表型遗传的遗传证据(双向杂交)以及提供的细胞分裂活性分析(流式细胞仪)表明,STK在种子发育的早期阶段作为母体生长激活剂起作用。此外,我们通过报告STK如何通过直接激活E2Fa(细胞周期的关键调节因子)的表达来积极调节细胞周期进展,从而描述了这种活性的分子机制。总而言之,我们的结果揭示了一个新的遗传网络,该网络有效地控制了拟南芥的种子大小。
更新日期:2021-01-20
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