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Multicenter Evaluation of an ELISA for the Detection of Cryptosporidium spp. Antigen in Clinical Human Stool Samples
Microorganisms ( IF 4.5 ) Pub Date : 2021-01-20 , DOI: 10.3390/microorganisms9020209
Romy Razakandrainibe , Célia Mérat , Nathalie Kapel , Marc Sautour , Karine Guyot , Gilles Gargala , Jean-Jacques Ballet , Patrice Le Pape , Frédéric Dalle , Loïc Favennec ,

Human cryptosporidiosis remains underdiagnosed, and rapid/accurate diagnosis is of clinical importance. Diagnosis of the Cryptosporidium oocyst in stool samples by conventional microscopy is labor-intensive, time-consuming, and requires skillful experience. Thus, we aimed to evaluate the usefulness of a coproantigen enzyme-linked immunosorbent assay (ELISA) test in detecting Cryptosporidium spp. from fecal specimens. For this aim, we evaluated the performances of a commercial ELISA (CoproELISA Cryptosporidium kit, Savyon Diagnostics, Israel) for the detection of Cryptosporidium spp. in random clinical stool samples through a multicenter study. The sensitivity and specificity for coproantigen ELISA were 98.86% and 94.32%, respectively. The coproantigen ELISA results indicate that the simple, rapid, reliable, and standardized immunoassay test is sensitive and specific for routine diagnosis, and may be useful for large-scale epidemiological studies of cryptosporidiosis.

中文翻译:

用于检测隐孢子虫的ELISA的多中心评估。临床人体粪便样本中的抗原

人类隐孢子虫病仍未得到充分诊断,因此快速/准确的诊断具有重要的临床意义。通过常规显微镜对粪便样品中隐孢子虫卵囊的诊断是费力的,费时的并且需要熟练的经验。因此,我们旨在评估辅助抗原酶联免疫吸附测定(ELISA)测试在检测隐孢子虫中的有用性。从粪便标本。为此,我们评估了用于检测隐孢子虫的商用ELISA(CoproELISA隐孢子虫试剂盒,Savyon Diagnostics,以色列)的性能。spp。通过多中心研究随机抽取临床粪便样本。coproantigen ELISA的灵敏度和特异性分别为98.86%和94.32%。辅助抗原ELISA结果表明,简单,快速,可靠和标准化的免疫测定测试对常规诊断敏感且特异,可能对隐孢子虫病的大规模流行病学研究有用。
更新日期:2021-01-20
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