Recently, several studies have highlighted the role of Citrus flavanones in counteracting oxidative stress and inflammatory response in bowel diseases. The aim of study was to identify the most promising Citrus flavanones by a preliminary antioxidant and anti-inflammatory screening by in vitro cell-free assays, and then to mix the most powerful ones in equimolar ratio in order to investigate a potential synergistic activity. The obtained flavanones mix (FM) was then subjected to in vitro simulated digestion to evaluate the availability of the parent compounds at the intestinal level. Finally, the anti-inflammatory activity was investigated on a Caco-2 cell-based model stimulated with interleukin (IL)-1β. FM showed stronger antioxidant and anti-inflammatory activity with respect to the single flavanones, demonstrating the occurrence of synergistic activity. The LC-DAD-ESI-MS/MS analysis of gastric and duodenal digested FM (DFM) showed that all compounds remained unchanged at the end of digestion. As proof, a superimposable behavior was observed between FM and DFM in the anti-inflammatory assay carried out on Caco-2 cells. Indeed, it was observed that both FM and DFM decreased the IL-6, IL-8, and nitric oxide (NO) release similarly to the reference anti-inflammatory drug dexamethasone.

中文翻译：

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体外模拟消化后柑桔类黄酮混合物的抗氧化和抗炎活性及其稳定性

最近，一些研究强调了柑橘类黄烷酮在抵抗肠道疾病中的氧化应激和炎症反应中的作用。研究的目的是确定最有前途的柑橘黄酮类化合物通过初步的抗氧化剂和抗炎药筛选进行体外无细胞试验，然后以等摩尔比混合最强大的黄酮类化合物，以研究潜在的协同活性。然后将获得的黄烷酮混合物（FM）进行体外模拟消化，以评估肠道水平上母体化合物的利用率。最后，在白介素（IL）-1β刺激的基于Caco-2细胞的模型上研究了抗炎活性。FM显示出相对于单一黄烷酮更强的抗氧化和抗炎活性，证明了协同活性的发生。胃和十二指肠消化FM（DFM）的LC-DAD-ESI-MS / MS分析表明，所有化合物在消化结束时均保持不变。作为证明，在对Caco-2细胞进行的抗炎实验中，FM和DFM之间观察到了叠加行为。实际上，已观察到，FM和DFM均降低了IL-6，IL-8和一氧化氮（NO）的释放，与参考抗炎药地塞米松相似。