Abstract

To characterize the metabolic versatility of denitrifying bacterial communities residing in the paddy soil, phospholipid fatty acid (PLFA) analyses were done along with nosZ encoding nitrous oxide reductase gene-based PCR-DGGE and real-time Q-PCR analysis. In this study, we have analyzed the abundance of nitrous oxide reductase (nosZ) genes and DGGE-based denitrifier community assessment was found to be correlated with soil PLFA profile in terms of their pattern analysis. Among all detected denitrifying bacteria, Ochrobactrum sp. represented a major fraction following Cupriavidus and uncultured bacteria. The data related to nosZ gene abundance were found to show similar pattern with PLFA-based lipid profile. Greater amount of lipid content (C18-C20) and denitrifiers diversity was apparently visible at Chandauli of Eastern UP, India and thus positively impact each other in the soil. Our study suggests that the nosZ-based DGGE and gene abundance data were increased in response to soil PLFA profiles. Subsequently, the presence of an increased lipid content of soil samples with increased abundance and diversity of denitrifying bacteria could be helpful in managing nutrient dynamics as well as microbial dynamics of paddy soil ecosystem.

摘要

为了表征稻田土壤中反硝化细菌群落的代谢多功能性，对磷脂脂肪酸（PLFA）进行了分析，同时编码基于一氧化二氮还原酶基因的nosZ进行了PCR-DGGE和实时Q-PCR分析。在这项研究中，我们分析了一氧化二氮还原酶（nosZ）基因的丰度，并且基于DGGE的反硝化器群落评估被发现与土壤PLFA谱相关联，涉及其模式分析。在所有检测到的反硝化细菌中，Ochrobactrum sp.。代表了铜杯和未培养细菌之后的主要部分。与nosZ相关的数据发现基因丰度与基于PLFA的脂质谱显示相似的模式。在印度东部东部的Chandauli，明显可见大量的脂质含量（C18-C20）和反硝化剂多样性，因此在土壤中彼此产生积极影响。我们的研究表明，基于nosZ的DGGE和基因丰度数据随着土壤PLFA谱的增加而增加。随后，土壤样品中脂质含量的增加以及反硝化细菌的丰度和多样性的增加可能有助于管理稻田土壤生态系统的养分动态和微生物动态。