C‐glycosylflavonoids are polyphenolic phytochemicals that are ubiquitously distributed in the plant kingdom and confer a wealth of health‐promoting benefits to human body. These compounds harbor one or more sugar moieties that are attached to various regions of the flavonoid scaffold through rigid C–C glycosidic bonds. The diverse structures of C‐glycosylflavonoids are granted by the prominent reactions of flavonoid C‐glycosyltransferases (CGTs). This review gives an extensive overview of CGTs that are involved in the biosynthesis of C‐glycosylflavonoids, focusing on their evolutionary relationship and substrate specificity. Furthermore, structural insights into the catalytic mechanism and protein engineering of flavonoid CGTs are summarized. This review provides an important guidance for identification of novel flavonoid CGTs, biosynthesis of C‐glycosylflavonoids, and elucidation of the function‐structure relationship of flavonoid CGTs.

中文翻译：

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类黄酮C-糖基转移酶：功能，进化关系，催化机制和蛋白质工程。

C-糖基类黄酮是在植物界广泛分布的多酚类植物化学物质，可为人体带来大量促进健康的益处。这些化合物带有一个或多个糖部分，这些糖部分通过刚性的C-C糖苷键连接到类黄酮支架的各个区域。的不同结构Ç -glycosylflavonoids由类黄酮的突出反应授予Ç -glycosyltransferases（CGTs）。本文综述了所涉及到的生物合成CGTs的广泛概述Ç-糖基类黄酮，着重于它们的进化关系和底物特异性。此外，总结了对类黄酮CGT的催化机理和蛋白质工程的结构见解。这篇综述为鉴定新型类黄酮CGT，生物合成C-糖基类黄酮以及阐明类黄酮CGT的功能-结构关系提供了重要指导。