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Hypermethylation of the PZP gene is associated with hepatocellular carcinoma cell proliferation, invasion and migration
FEBS Open Bio ( IF 2.6 ) Pub Date : 2021-01-20 , DOI: 10.1002/2211-5463.13093
Minhua Wu 1 , Hui Lan 1 , Zhongwei Ye 1 , Yonghui Wang 1
Affiliation  

Pregnancy zone protein (PZP), a member of the proteinase inhibitor I39 (‐2‐macroglobulin) family of proteins, is involved in the initiation and development of various tumors. The gene encoding PZP is hypermethylated and expressed at low levels in hepatocellular carcinoma (HCC) tissue and cells, but the function of PZP in HCC cells remains unclear. Here, we analyzed DNA methylation and mRNA expression of HCC in The Cancer Genome Atlas Liver Hepatocellular Carcinoma dataset. We identified 10 methylation‐driven genes, of which PZP was significantly hypermethylated and poorly expressed in tumor tissue. We confirmed that PZP is highly methylated and poorly expressed in HCC cell lines via quantitative real‐time PCR experiment and methylation‐specific PCR. Furthermore, PZP markedly inhibited the proliferation, invasion and migration of HCC cells. These findings may provide a basis for exploring novel therapeutic targets for HCC.

中文翻译:

PZP基因的高甲基化与肝细胞癌细胞增殖、侵袭和迁移有关

妊娠区蛋白 (PZP) 是蛋白酶抑制剂 I39(-2-巨球蛋白)家族的成员,参与各种肿瘤的发生和发展。编码 PZP 的基因在肝细胞癌 (HCC) 组织和细胞中被高甲基化并低水平表达,但 PZP 在 HCC 细胞中的功能尚不清楚。在这里,我们分析了癌症基因组图谱肝细胞癌数据集中 HCC 的 DNA 甲基化和 mRNA 表达。我们鉴定了 10 个甲基化驱动的基因,其中PZP显着高甲基化且在肿瘤组织中表达不佳。我们确认PZP通过定量实时 PCR 实验和甲基化特异性 PCR,在 HCC 细胞系中高度甲基化和低表达。此外,PZP 显着抑制 HCC 细胞的增殖、侵袭和迁移。这些发现可能为探索 HCC 的新治疗靶点提供基础。
更新日期:2021-03-04
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