Exploitation of tuff deposits represents an intensive and destructive human activity that can completely modify natural soils, sometimes leading to their total disappearance. Soil reclamation of quarry areas can usually be done by soil rebuilding, employing complex and expensive techniques. In this research, we aimed to evaluate reconstructed Technosols and associated vegetation, called pedotechnosystem (PTS) and their development and behaviour for tuff quarry reclamation for both environmental rehabilitation and agricultural purposes. Technosols were investigated under three different conditions: i) a control consisting of a constructed Technosol (CT) without additional treatments; ii) CT treated with conventional mineral fertilization (CTCF); and, iii) CT treated with a composted mix of commercial organic amendments (CTOA). Different open-air mesocosms (pots) were investigated and evaluated in terms of: i) development, production, and metabolomic profile of rosemary (Rosmarinus officinalis L.), olive (Olea europaea L.), and grapevine (Vitis Vinifera L. ssp. Sativa) in a mixture with a pasture species: ii) the Technosols physical-chemical responses. Specifically, after 15 months, all investigated PTS were evaluated in terms of: i) pasture species total dry matter (TDM) production, height (H), stem diameter (D) and nitrogen (N), phosphorus (P), and potassium (K) content of dry matter; ii) physical-chemical analyses of all investigated substrates; iii) olive, grape, and rosemary characterization by analysing N, P, and K content in dry leaves as well as their metabolomic profile. Statistical differences among treatments were ascertained through ANOVA, while observed variability was interpreted using a principal factor analysis (PFA). Use of CTOA resulted in statistically (p < 0.05) higher performances in terms of TDM as well as P contents in harvested pasture species. In CTOA, more favourable pH conditions (near neutral) for plant growth were observed; additionally, such PTS showed the best performances in terms of soil organic matter (SOM), total N, and P as well as an improvement for most of the investigated organic fractions and relative humification indexes. Identified metabolites varied greatly by species, underscoring the capability of metabolites in fingerprinting different species and related responses to environmental stress factors. Results from PFA showed that: i) CTOA produced better growing conditions and agronomic performance among all investigated PTS; ii) metabolomic profiles are a reliable fingerprint for investigating plant responses to environmental stress and can help differentiate behaviour that follows. Overall, the research demonstrated that CTOA represents a low-cost easily applicable technique for soil rebuilding in tuff quarry areas.

对凝灰岩沉积物的开采代表了人类的强烈破坏性活动，可以完全改变天然土壤，有时导致其完全消失。采石场的土壤开垦通常可以通过土壤复垦，采用复杂而昂贵的技术来完成。在这项研究中，我们旨在评估重建的Technosol和相关植被（称为pedotechnosystem（PTS））及其在凝灰采石场开垦中的发展和行为，以用于环境修复和农业用途。在三种不同条件下对甲酚进行了研究：i）对照物，由未经处理的人造甲酚（CT）组成；ii）用常规矿物肥料（CTCF）处理过的CT；并且，iii）用商业有机改良剂（CTOA）的混合肥料处理过的CT。研究和评估了不同的露天中观（锅）：i）迷迭香（Rosmarinus officinalis L.），橄榄（Olea europaea L.）和葡萄（Vitis Vinifera L. ssp ）的发育，生产和代谢组学特征（Sativa）与牧草种类的混合物：ii）Technosols的物理化学反应。具体而言，在15个月后，根据以下各项对所有调查的PTS进行评估：i）牧草物种总干物质（TDM）产量，高度（H），茎直径（D）和氮（N），磷（P）和钾（K）干物质含量；ii）所有被调查底物的物理化学分析；iii）通过分析干叶中N，P和K的含量及其代谢组学特征来表征橄榄，葡萄和迷迭香的特性。通过方差分析确定治疗之间的统计差异，同时使用主因子分析（PFA）解释观察到的变异性。在收获的牧场物种中，使用CTOA在TDM和P含量方面具有统计学上的优势（p <0.05）。在CTOA中，观察到了更有利于植物生长的pH条件（接近中性）。此外，这类PTS在土壤有机质（SOM），总氮和磷方面表现出最佳性能，并且对大多数研究的有机组分和相对增湿指数都有改进。鉴定出的代谢物因物种而异，强调了代谢物对不同物种进行指纹识别的能力以及对环境压力因素的相关反应。PFA的结果表明：i）CTOA在所有调查的PTS中均产生了更好的生长条件和农艺性能；ii）代谢组学谱是研究植物对环境胁迫反应的可靠指纹，可帮助区分随后的行为。总体而言，研究表明CTOA是凝灰岩采石场土壤修复的一种低成本，易于应用的技术。