Emulsification and biological souring of alkaline-surfactant-polymer (ASP) flooding produced water constitute two serious issues currently facing the petroleum industry. This study introduced a potential method to solve these two issues synergistically. Nitrate-reducing bacteria (NRB) with alkane-degrading and demulsifying ability were isolated, and their potential and feasibility in the demulsification and bio-souring control were evaluated. The results indicated that carbon sources played decisive roles not only in bio-demulsifying performance and mechanisms but also in the lasting potential of bio-souring control. The sodium acetate induced bio-demulsifying bacteria, Gordonia sp. TD-4, to separate emulsified oil by bio-flocculation-derived demulsification, while oil-soluble carbon sources like paraffin oil induced TD-4 to separate emulsified oil by competitive adsorption-derived demulsification. TD-4 could effectively decrease the concentration of emulsified crude oil in ASP flooding produced water (oil in water emulsion, O/W; initial oil concentration 965 ± 21 mg/L; mean oil droplet diameter 65 nm) to less than 40 mg/L. The potential of electronic donor competition and nitrite accumulation indicated TD-4 could be a bio-augmenter to control bio-souring.

碱性表面活性剂聚合物（ASP）驱采采出水的乳化和生物酸化是目前石油工业面临的两个严重问题。这项研究介绍了一种潜在的方法来协同解决这两个问题。分离出具有烷烃降解和破乳能力的硝酸盐还原菌（NRB），并评价其在破乳和生物发酵控制中的潜力和可行性。结果表明，碳源不仅在生物破乳性能和机理中起决定性作用，而且在生物酸控制的持久潜力中也起决定性作用。乙酸钠诱导的生物破乳细菌戈登尼亚sp。TD-4可通过生物絮凝衍生的破乳作用分离乳化油，而油溶性碳源（如石蜡油）则可通过竞争性吸附衍生的破乳作用诱导TD-4分离乳化油。TD-4可以有效地降低ASP驱采水中的乳化原油的浓度（水包油乳状液，O / W；初始油浓度965±21 mg / L；平均油滴直径65 nm）至小于40 mg / L. 电子供体竞争和亚硝酸盐积累的潜力表明TD-4可能是控制生物变质的生物增强剂。