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Evaluation of parameters to characterise germination-competent mature somatic embryos of Norway spruce (Picea abies)
Biosystems Engineering ( IF 5.1 ) Pub Date : 2021-01-20 , DOI: 10.1016/j.biosystemseng.2020.12.013
Kim-Cuong Le , Aruna B. Weerasekara , Sonali S. Ranade , E-M Ulrika Egertsdotter

Somatic embryogenesis (SE) is an in vitro clonal propagation method increasingly in use for large scale clonal propagation of conifer plants. However, asynchronous development of immature somatic embryos into mature embryos lowers the yield of plants and limits the benefits for industrial applications. Through the development of an automated harvesting system (SE Fluidics system) for singulation, imaging and selection of mature embryos at the correct developmental stages, yields can be improved. Image analysis software developed for use with the SE Fluidics system has been utilised to characterize and sort mature embryos according to their morphological measurements related to developmental capacity. Here a set of imaging data from the SE Fluidics system has been analysed with the goal of further refining the selection criteria for germination-competent mature embryos.



中文翻译:

评价表征挪威云杉(Picea abies)萌发能力的成熟体细胞胚的参数

体细胞胚发生(SE)是一种体外克隆繁殖方法,越来越多地用于针叶树植物的大规模克隆繁殖。然而,未成熟的体细胞胚异步发育成成熟胚降低了植物的产量并限制了工业应用的益处。通过开发自动收获系统(SE Fluidics系统)以在正确的发育阶段对成熟胚进行单颗化,成像和选择,可以提高产量。已开发用于SE Fluidics系统的图像分析软件已根据与发育能力相关的形态学特征对成熟的胚胎进行表征和分类。

更新日期:2021-01-20
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