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Identification of main and epistatic QTLs and QTL through environment interactions for eating and cooking quality in Iranian rice
Euphytica ( IF 1.9 ) Pub Date : 2021-01-20 , DOI: 10.1007/s10681-020-02759-8
Hossein Rahimsoroush , Farhad Nazarian-Firouzabadi , Maryam Hosseini Chaloshtari , Ahmad Ismaili , Ali Akbar Ebadi

Breeding new rice varieties with high Eating and Cooking Quality (ECQ) characteristics is an important goal in rice breeding programs. To this end, 242 Recombinant Inbred Lines (RILs) of two populations derived from Alikazemi/IR67017-180-2-1-2 and Alikazemi/Saleh crosses were evaluated in two environments. The main and epistatic QTLs and QTL × Environment (Q × E) interactions were analyzed using the Mixed-Model-based Composite Interval Mapping method. A total of 17 main QTLs with additive effects were mapped on 6 rice chromosomes, including 6 QTLs for Amylose Content, 5 QTLs for Gelatinization Temperature and 6 QTLs for Gel Consistency. Seven QTLs were found on chromosome 6 associated with a common RM4128 marker (close to the Alk locus) influencing the ECQ characteristics with favorable alleles from the common female parent. Five QTL clusters were identified on chromosome 6, 7, 8, and 9, suggesting that the co-located QTLs with pleiotropic effects may control ECQ traits. A major QTL cluster was found for AC and GC between RM4128 and RM549 markers close to the Alk locus on chromosome 6, which is in the vicinity of another QTL located in RM587-RM4128 interval. 13 QTL pairs were identified to have a significant epistatic, Additive × Additive effects involved in controlling ECQ traits. A small phenotypic variation was found for the epistatic QTLs and Q × E interactions, suggesting the complexity of ECQ inheritance traits in rice. Further comprehensive studies and validation of the detected QTLs could be useful for marker-assisted selection to improve grain quality in rice.



中文翻译:

通过环境相互作用识别主要和上位QTL和QTL,以改善伊朗大米的饮食质量

具有高饮食和烹饪质量(ECQ)特征的新水稻品种的育种是水稻育种计划的重要目标。为此,在两个环境中评估了来自Alikazemi / IR67017-180-2-1-2和Alikazemi / Saleh杂交的两个种群的242个重组自交系(RIL)。使用基于混合模型的复合区间映射方法分析了主要和上位QTL和QTL×环境(Q×E)的相互作用。共有17个具有加性作用的主要QTL定位在6个水稻染色体上,其中包括6个直链淀粉含量的QTL,5个糊化温度的QTL和6个凝胶一致性的QTL。在6号染色体上发现了7个QTL,它们与一个共同的RM4128标记相关(靠近Alk)位点)会影响来自普通雌性亲本的有利等位基因,从而影响ECQ特征。在6号,7号,8号和9号染色体上鉴定出5个QTL簇,表明具有多效性的共居QTL可能控制ECQ性状。在靠近Alk的RM4128和RM549标记之间发现了一个主要的AC和GC QTL簇6号染色体上的基因座,位于另一个位于RM587-RM4128区间的QTL附近。鉴定出13个QTL对具有控制ECQ性状的显着上位性,加和加作用。发现上位QTL和Q×E相互作用的表型变化很小,表明水稻ECQ遗传性状的复杂性。进一步的综合研究和检测到的QTL的验证可能对标记辅助选择以改善稻米品质有帮助。

更新日期:2021-01-20
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