Trichloroethylene (TCE) is one of the most common groundwater pollutants. It is carcinogenic, teratogenic, mutagenic and poses a serious threat to human health and the environment. Therefore, reducing the environmental toxicity of TCE is of great significance. Anaerobic sludge was cultured and acclimated in an upflow anaerobic sludge blanket (UASB) reactor in this study. The Chemical Oxygen Demand (COD) concentration of the influent was approximately 2500 mg L⁻¹, and the TCE concentration of the influent ranged from 1.46 mg L⁻¹ to 73 mg L⁻¹. After biodegradation of the anaerobic microflora, the COD removal rate was approximately 85%, and the TCE removal rate was over 85%. The microbial community of anaerobic sludge was analysed by 16 S rDNA clone libray and 454 high-throughput sequencing. Through analysis of the sequencing results, we found that there were a variety of acid-forming bacteria, anaerobic dechlorinating bacteria, and methanogenic bacteria. Based on the analysis of microflora function, it was speculated that the TCE metabolic pathway took place in UASB reactors. Desulfovibrio and Syntrophobacter provided an anaerobic environment, and acid-forming bacteria metabolise organic compounds into hydrogen. With Dehalobacter and Geobacter, TCE as an electron acceptor is dechlorinated and reduced under the anaerobic environment, in which hydrogen acts as an electron donor. By this, we clarified the metabolic pathway for improving TCE bioremediation.

三氯乙烯 (TCE) 是最常见的地下水污染物之一。它具有致癌性、致畸性、致突变性，对人类健康和环境构成严重威胁。因此，降低三氯乙烯的环境毒性意义重大。在本研究中，厌氧污泥在上流式厌氧污泥床 (UASB) 反应器中培养和驯化。进水的化学需氧量 (COD) 浓度约为 2500 mg L ^-1，进水的 TCE 浓度范围为 1.46 mg L ^-1至 73 mg L ^-1. 厌氧菌群经生物降解后，COD去除率约85%，TCE去除率超过85%。采用16S rDNA克隆文库和454高通量测序对厌氧污泥微生物群落进行分析。通过对测序结果的分析，我们发现存在多种产酸菌、厌氧脱氯菌和产甲烷菌。基于菌群功能分析，推测三氯乙烯代谢途径发生在UASB反应器中。Desulfovibrio和Syntrophobacter提供了一个厌氧环境，产酸细菌将有机化合物代谢成氢气。随着脱卤和地杆菌, 作为电子受体的 TCE 在厌氧环境下被脱氯和还原，其中氢作为电子供体。由此，我们阐明了改善 TCE 生物修复的代谢途径。